A STUDY OF THE DISTRIBUTION OF AXONS CONTAINING SUBSTANCE P AND THE LOCALIZATION OF NEUROKININ-1 RECEPTOR IN TEMPOROMANDIBULAR JOINT ARTHRITIS OF THE RAT.

大鼠颞下颌关节关节炎中 P 物质轴突的分布和 NEUROKININ-1 受体的定位研究。

• 批准号: 09832010
• 负责人: TSUKIYAMA Yoshihiro
• 金额: $ 1.98万
• 依托单位: KYUSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09832010/
• 关键词: TEMPOROMANDIBULAR JOINT; RAT; ADJUVANT-INDICED ARTHRITIS; SYNOVIUM; NITRIC OXIDE SYNTHASE; IMMUNOHISTOCHEMISTRY; IMMUNOCYTOCHEMISTRY

1. Immunolight- and immunoelectron-microscopic observation of the localization of NOSs in the normal rat TMJ : Six-week-old male Wistar rats were anesthetized and perfused transcardically with fixative, and TMJs were dissected out. After decalcification, frozen sections were obtained and submitted to immunolight- and immunoelectron-microscopic observation. ABC method with antibodies for eNOS or iNOS was utilized. Immunoreactivity for eNOS and iNOS was found in the synovial lining cells in the anterior and posterior portions of the synovium facing the upper compartment of the TMJ cavity, and in the anterior and lateral portions of the synovium facing of the lower compartment. eNOS was also detected in the endothelial cells of blood vessels close to the synovium. Immunoelectron-microscopic observation revealed that immunoreactivity for eNOS and iNOS was mainly found in the type A cells of the synovial lining cells. In these type A cells, immunoreactive products for eNOS and iNOS were distributed mainly in the cytoplasm, and in the vesicular and vacuolar structures.2. Immunolight-microscopic observation of the localization of NOSs in the rat TMJ with adjuvant-induced arthritis : Arthritis was induced by the injection of the adjuvant to 7-week-old male Lewis rats. immunolight-microscopic observation for eNOS and iNOS was performed in the same manner as the above mentioned method. The localization of immunoreactivity for eNOS and iNOS in the synovial lining cells of the inflamed TMJ was similar to that of the normal joint. Immunoreactivity for eNOS or iNOS was found in proliferated synovial lining cells facing the upper or lower joint compartment. The strength of immunoreactivity for eNOS was, however, stronger than that for iNOS.Although no clear difference was found in the strength of immunoreactivity for eNOS in the synovial lining cells between the normal rat and the arthritis rat, immunoreactivity for iNOS was reduced in the arthritis rat.

1。对正常大鼠TMJ中NOSS定位的免疫光和免疫电子显微镜观察：六周大的雄性Wistar大鼠被麻醉，并用固定剂透露蛋白质，并解散了TMJ。脱钙后，获得冷冻切片并提交给免疫光和免疫电子显微镜观察。使用了具有eNOS或iNOS抗体的ABC方法。在面向TMJ腔的上部室的前部和后部的滑膜衬细胞中发现了eNOS和INOS的免疫反应性，在TMJ腔的上部，以及下部隔室面向的滑膜前部和外侧部分。还在靠近滑膜的血管内皮细胞中检测到eNOS。免疫电子显微镜观察表明，eNOS和INOS的免疫反应性主要在滑膜衬里细胞的A型细胞中发现。在这些类型的A细胞中，用于eNOS和INOS的免疫反应性产物主要分布在细胞质中，以及囊泡和液泡结构中。2。免疫光显微镜观察NOSS在大鼠TMJ中与辅助性关节炎的定位：通过将辅助药物注射至7周大的雄性路易斯大鼠，引起关节炎。对eNOS和INOS的免疫光显微镜观察的方式与上述方法相同。发炎的TMJ的滑膜衬里细胞中eNOS和iNOS免疫反应性的定位与正常关节相似。在面向上或下关节室的增殖滑膜细胞中发现了eNOS或iNOS的免疫反应性。然而，eNOS免疫反应性的强度比INOS的强度更强。尽管在正常大鼠和关节炎大鼠之间的滑膜衬里细胞中eNOS的免疫反应强度尚无明显差异，但关节炎大鼠的INOS的免疫反应性降低。