study of Proteoglycans in Brains of Elderly Humans

老年人脑中蛋白多糖的研究

• 批准号: 08838024
• 负责人: SHIMADA Hiroyuki
• 金额: $ 1.09万
• 依托单位: Tokyo Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08838024/
• 关键词: Aging brain; Proteoglycans; Immunostains; Alzheimer's disease; アルツハイマー病; 免疫組織化学的染色

Immunoactivities for antibody to HSPG were clearly demonstrated in classical plaques of NPs containing a central amyloid core and also in primitive plaques without an amyloid core. The 'amorphous' or 'diffuse' plaques were also strongly HSPG-immunopositive. In addition, HSPG-immunopositivity was almost always observed even in meningeal and intracerebral small vessels/capillaries which did not contains amyloid. Immunostaining of four other monoclonal antibodies (DSPG : 6-B-6, CSPG : CS-56, Di-4S : 2-B-6, Di-6S : 3-B-3) was limited to two types of Nps (classical and primitive types). Immunoactivity of DSPG and CSPG was easily identified in glial cells and extracellular matrix of normal aged and ADA brains. HSPG,DSPG and CSPG were immunohistochemically detected in both intracellular and extracellular (ghost) NFTs and nontangle-bearing neurons in normal aged brains and the AD brain. Immunoreaction with HSPG was strongly immunopositive in all three types of NPs (diffuse, primitive and classical types of NPs) in contrast to the four other antibodies. However, no distinct difference in immunoreactive intensity was sen in the AD brain compared to normal aged brains.

在含有中央淀粉样蛋白核的NP的经典斑块中清楚地证明了对HSPG抗体的免疫活性，也证明了无淀粉样蛋白核的原始斑块。 “无定形”或“弥漫性”斑块也非常强烈HSPG免疫阳性。此外，即使在不包含淀粉样蛋白的脑膜和脑脑脑和小血管/毛细管中，HSPG - 免疫阳性也几乎总是观察到。其他四种单克隆抗体的免疫染色（DSPG：6-B-6，CSPG：CS-56，DI-4S：2-B-6，DI-6S：3-B-3）仅限于两种NPS（经典）和原始类型）。 DSPG和CSPG的免疫活性在神经胶质细胞以及正常老年和ADA大脑的细胞外基质中很容易鉴定。 HSPG，DSPG和CSPG在正常老年大脑和AD大脑的细胞内和细胞外（幽灵）NFT和非义神经元中都在免疫组织化学上检测到。与其他四种抗体相反，HSPG的免疫反应在所有三种NP（弥漫性，原始和经典的NPS）中都具有强烈的免疫阳性。然而，与正常老化的大脑相比，AD脑的免疫反应强度没有明显差异。

项目成果

Tsuchiya, K., and Shimada, H.: "Dentorubra $ pallidoluysian atrophy(DRPLA)-Clinicopathological study of eight autopsy cases with special refence to the clinicopathological correlation of pyramidal signs and involvement of the pyramidal tract" Neuropatholo

Tsuchiya, K. 和 Shimada, H.：“Dentorubra $ pallidoluysian 萎缩症 (DRPLA) - 八个尸检病例的临床病理学研究，特别涉及锥体体征和锥体束受累的临床病理学相关性”

Saido, T.Iwatsudo, T., Ihara, Y., and Shimada, H.etc: "Dominant and Dillerential Deposition of Distinct beta-amyloid Peptide Species, A-betaN3 (pE) in Senile Plaques :" Neuron. 14. 457-466 (1995)

Saido, T.Iwatsudo, T.、Ihara, Y. 和 Shimada, H.等：“老年斑块中不同 β-淀粉样肽种类 A-betaN3 (pE) 的显性和差异沉积：”神经元。

Shimada, H.Noda, K., Mori, M., Aoki, N., Tajima, M., and Kato, K.: "Papovavirus detection by electron microscopy in the brain of an elderly patient without overt progressive multifocal encephalopathy :" Vichow Archiv. 424. 569-572 (1994)

Shimada, H.Noda, K.、Mori, M.、Aoki, N.、Tajima, M. 和 Kato, K.：“通过电子显微镜在无明显进展性多灶性脑病的老年患者大脑中检测乳多病毒：”

Yoshikawa, M., Shimada, H.,: "Construcion and evaluation of a virG thyA double mutant of shigella flexneri 2 a as candidata live-attenuated oral vaccine" Vaccine. 13. 1436-1440 (1995)

Yoshikawa, M., Shimada, H.，：“福氏志贺氏菌 2a virG thyA 双突变体作为候选减毒口服疫苗的构建和评估”疫苗。

Tsuchiya, K., Ozawa, E., Saito, H., Irie, H., and Mizutani, T.: "Neurc pathology of Iate cortical change on an autopsy case and review of Japanese cases" European Neurology. 34. 253-262 (1994)

Tsuchiya, K.、Ozawa, E.、Saito, H.、Irie, H. 和 Mizutani, T.：“尸检病例中晚期皮质变化的神经病理学和日本病例回顾”《欧洲神经病学》。