Microbioassay for the analysis of toxic factors produced by red tide phytoplankton

赤潮浮游植物有毒因子分析的微生物测定法

• 批准号: 23580255
• 负责人: ODA Tatsuya
• 金额: $ 3.41万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23580255/
• 关键词: プランクトン; 赤潮; 毒性; 培養細胞; バイオアッセイ; 細胞培養

Harmful algal blooms (HABs) are one of serious environmental problems. To investigate the toxic mechanism of HABs species, direct exposure experiments against fish or shellfish have been conducted. However, such exposure experiments generally require special animal rearing facilities and mass-culture of phytoplankton. Usage of appropriate cultured cells as an alternative for whole living animals seems to be a promising strategy. We evaluated lactate dehydrogenase (LDH)-release assay as a simple estimation method for toxic potential of harmful algal species at cellular level. Live cell suspension of Karenia mikimotoi (strain SUO-1), which is highly toxic to rotifer and has potent hemolytic activity, induced the release of significant levels of LDH from all the cell lines tested in cell-density and time-dependent manners, whereas another strain of K. mikimotoi (strain FUK), which is less-toxic to rotifer and has low hemolytic activity, showed fairly low activities.

有害藻华（HAB）是严重的环境问题之一。为了研究HABs物种的毒性机制，对鱼类或贝类进行了直接暴露实验。然而，此类暴露实验通常需要特殊的动物饲养设施和浮游植物的大规模培养。使用适当的培养细胞作为整个活体动物的替代品似乎是一个有前途的策略。我们评估了乳酸脱氢酶（LDH）释放测定作为细胞水平上有害藻类毒性潜力的简单估计方法。 Karenia mikimotoi（菌株 SUO-1）的活细胞悬浮液对轮虫具有高毒性并具有强效溶血活性，诱导以细胞密度和时间依赖性方式测试的所有细胞系释放显着水平的 LDH，而另一种 K. mikimotoi 菌株（FUK 菌株）对轮虫毒性较小，溶血活性也较低，但活性相当低。

项目成果

The potent activity of sulfated polysaccharide, ascophyllan, isolated from Ascophyllum nodosum to induce nitric oxide and cytokine production from mouse macrophage RAW264.7 cells : Comparison between ascophyllan and fucoidan

从泡叶藻中分离出来的硫酸化多糖泡叶素诱导小鼠巨噬细胞 RAW264.7 细胞产生一氧化氮和细胞因子的有效活性：泡叶素和岩藻依聚糖的比较

• 发表时间: 2011
• 作者: Z. Jiang; T. Okimura; K. Yamaguchi;T. Oda
• 通讯作者: T. Oda

The potent activity of sulfated polysaccharide, ascophyllan, isolated from Ascophyllum nodosum to induce nitric oxide and cytokine production from mouse macrophage RAW264.7 cells: Comparison between ascophyllan and fucoidan.

从泡叶藻中分离出来的硫酸化多糖泡叶聚糖诱导小鼠巨噬细胞 RAW264.7 细胞产生一氧化氮和细胞因子的有效活性：泡叶聚糖和岩藻依聚糖的比较。

• DOI: 10.1016/j.niox.2011.10.001
• 发表时间: 2011
• 作者: Zedong Jiang; Takasi Okimura; Kenichi Yamaguchi;Tatsuya Oda.
• 通讯作者: Tatsuya Oda.

Evaluation of LDH-release Assay for the Detection and Analysis of Cellular Level Toxic Potential of HAB species

用于检测和分析 HAB 物种细胞水平毒性潜力的 LDH 释放测定法的评估

• 发表时间: 2012
• 作者: Yanan Zou; Daekyung Kim; Yasuhiro Yamasaki; Kenichi Yamaguchi; Tatsuya Oda
• 通讯作者: Tatsuya Oda

Evaluation of toxic potential of newly isolated Chattonella antiqua, by laboratory exposure experiments and micro-bioassay using cultured cells

通过实验室暴露实验和使用培养细胞的微生物测定来评估新分离的古查托氏菌的毒性潜力

• 发表时间: 2012
• 作者: Junya Tomita; Tomoki Nishiguchi; Motoaki Yagi; Daekyung Kim;Tatsuya Oda
• 通讯作者: Tatsuya Oda

Evaluation of LDH-release Assay for the Detection and Analysis of Cellular Level Toxic Potential of HAB species

用于检测和分析 HAB 物种细胞水平毒性潜力的 LDH 释放测定法的评估

• 发表时间: 2012
• 作者: Yanan Zou; Daekyung Kim; Yasuhiro Yamasaki; Kenichi Yamaguchi; Tatsuya Oda
• 通讯作者: Tatsuya Oda