Comparative study on the toxic factors of Chochlodinium polykrikoides and Chattonella marina

多环藻与海藻的毒性因素比较研究

• 批准号: 20580204
• 负责人: ODA Tatsuya
• 金额: $ 3万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20580204/
• 关键词: プランクトン; 赤潮; 毒性; 活性酸素; 鞭毛藻類; 粘液

Generation of reactive oxygen species (ROS) by Chattonella marina could be confirmed by ESR spin trapping method. Immunohistochemical analysis of gill lamellae from yellowtail exposed to C.marina using antiserum against crude glycocalyx demonstrated that there were the antigns recognaised by the antiserum on the surface of gill lamellae. As compared to C.marina, the levels of ROS detected in Cochlodinium polykrikoides were trace levels. Furthermore, no significant increase in O^2_- generation by C.polykrikoides was observed in the presence of lectins or fish mucus prepared from skin and gill of yellowtail, whereas C.marina generated increased level of O^2_- responding to these stimuli. The cell-free aqueous extract prepared from C.polykrikoides showed toxic effect on HeLa cells, but the extract of C.marina had almost no effect. Furthermore, gradual accumulation of polysaccharides in the medium was observed during the culture of C.polykrikoides, but no such change was observed in the medium of C.marina. These results suggest that ROS may play a significant role in the fish-killing activity of C.marina, whereas in the case of C.polykrikoides, certain toxic substances or polysaccharides are mainly responsible for the toxicity rather than ROS.

可以通过ESR自旋捕获方法证实Chattonella marina产生活性氧(ROS)。使用针对粗糖萼的抗血清对暴露于C.marina的黄尾鱼的鳃片进行免疫组织化学分析，证明在鳃片表面存在抗血清识别的抗原。与 C.marina 相比，Cochlodinium polykrikoides 中检测到的 ROS 水平为痕量水平。此外，在凝集素或从黄尾鱼的皮肤和鳃制备的鱼粘液存在的情况下，没有观察到 C.polykrikoides 产生的 O^2_- 显着增加，而 C.marina 响应这些刺激产生了增加的 O^2_- 水平。 C.polykrikoides制备的无细胞水提取物对HeLa细胞有毒性作用，而C.marina提取物几乎没有作用。此外，在C.polykrikoides的培养过程中，观察到培养基中多糖逐渐积累，但在C.marina的培养基中没有观察到这种变化。这些结果表明，ROS可能在C.marina的杀鱼活性中发挥重要作用，而就C.polykrikoides而言，某些有毒物质或多糖是造成毒性的主要原因，而不是ROS。

项目成果

Possible involvement of hemolytic activity in the contact-dependent lethal effects of the dinoflagellate Karenia mikimotoi on the rotifer Brachionus plicatilis.

甲藻 Karenia mikimotoi 对轮虫 Brachionus plicatilis 的接触依赖性致死作用可能涉及溶血活性。

• 发表时间: 2010
• 作者: Y.Zou; Y.Yamasaki; Y.Matsuyama; K.Yamaguchi; T.Honjo; T.Oda
• 通讯作者: T.Oda

Evaluation of the potential biological toxicities of aqueous extracts from red tide phytoplankton cultures in in vitro and in vivo systems.

评估赤潮浮游植物培养物水提取物在体外和体内系统中的潜在生物毒性。

• 发表时间: 2010
• 作者: D.Kim; S.H.Cha; E.Sato; Y.Niwano; M.Kohno; Z.Jiang; Y.Yamasaki; Y.Matsuyama; K.Yamaguchi; T.Oda
• 通讯作者: T.Oda

The possibility of reactive oxygen species (ROS)-independent toxic effects of Cochlodinium polykrikoides on damselfish (Chromis caerulea).

Cochlodinium polykrikoides 对雀鲷 (Chromis caerulea) 产生与活性氧 (ROS) 无关的毒性作用的可能性。

• 发表时间: 2009
• 作者: D.Kim; Y.Yamasaki; T.Yamatogi; K.Yamaguchi; Y.Matsuyama; Y.
• 通讯作者: Y.

Biological activities of fucose-containing polysaccharide ascophyllan isolated from the brown alga Ascophyllum nodosum.

从褐藻泡叶藻中分离的含岩藻糖多糖泡叶素的生物活性。

• 发表时间: 2009
• 作者: S.Nakayasu; R.Soegima; K.Yamaguchi; T.Oda
• 通讯作者: T.Oda

The possibility of reactive oxygen species(ROS)-independent toxic effects of Cochlodinium polykrikoides on damselfish(Chromis caerulea).

Cochlodinium polykrikoides 对雀鲷 (Chromis caerulea) 产生不依赖于活性氧 (ROS) 的毒性作用的可能性。

• 发表时间: 2009
• 作者: D.Kim; Y.Yamasaki; T.Yamatogi; K.Yamaguchi; Y.Matsuyama; Y.
• 通讯作者: Y.