Mechanism and significance of generation of reactive oxygen by red tide plankton, Chattonella

赤潮浮游生物查托氏菌产生活性氧的机制及意义

基本信息

批准号：
07660251
负责人：
ODA Tatsuya
金额：
$ 1.28万
依托单位：
Nagasaki University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07660251/
关键词：
Red Tide Plankton Reactive Oxygen Species Toxicity Chattonella Raphyidophyceae

项目摘要

Chattonella marina, a raphidophycean flagellate, is one of the most noxious red tide phytoplankton and is highly toxic to fish, especially to yellowtail Seriola quinqueradiata. Although the precise mechanism of the toxic action or Chattonella sp.remains unclear, it has been demonstrated that a decrease in oxygen partial pressure or arterial blood is the earliest physiological disturbance observed in fish after exposure to C.marina. Physiological and histological studies of fish exposed to C.marina also suggested that the blockade of respiratory water flow through the gill lamellae caused by excessive mucus interferes with O2 transfer, resulting in asphyxia. Recent studies have shown that Chattonella sp.generates reactive oxygen species (ROS) such as O2-, H2O2, and hydroxyl radical (OH). Furthermore, it has been reported that Heterosigma akashiwo, which is also classified in a raphidophycean, produces ROS,and showed an oxygen-radical-mediated toxic effect on rainbow trout, Oncorhynchus … More mykiss. Thus, it is likely that the production of ROS is a common feature of raphidophycean flagellates. This notion is supported by our recent findings in which other raphidophycean flagellates such as Olisthodiscus luteus and Fibrocapsa japonica produce O2-and H2O2. Since harmful effects or oxygen radicals have been well documented. ROS generated by Chattonella sp.may be responsible for gill tissue injury. Interestingly, resent results domonstrated that the generation of ROS by Chattonella is stimulated by lectins such as Con A,CBH,and WGH,suggesting that cell-surface redox enzyme may be involved in O2-production, and such enzyme is responsible for the lectin-stimulation. Furthermore, Fish mucus prepared from skin and gill of yellowtail, red sea bream, and japanese flounder stimulated C.marina and H.akashiwo to generate increased amounts or O2-in a concentration dependent manner. These results suggest that fish mucus enhances the ROS mediated toxic effects of Chattonella against fish. Less

查托氏菌（Chattonella marina）是一种针藻类鞭毛虫，是最毒的赤潮浮游植物之一，对鱼类，尤其是黄尾鰤具有剧毒，尽管查托氏菌的毒性作用的确切机制仍不清楚，但已证明，氧分压或动脉血下降是鱼类接触 C.marina 后观察到的最早的生理紊乱。 C.marina 还认为，过多的粘液会阻碍通过鳃片的呼吸水流，从而干扰 O2 转移，从而导致窒息。最近的研究表明，查托氏菌会产生活性氧 (ROS)，例如 O2-、H2O2。此外，据报道，也属于针藻类的异形藻（Heterosigma akashiwo）会产生 ROS，并表现出活性。氧自由基介导的对虹鳟鱼、Oncorhynchus ... More mykiss 的毒性作用因此，ROS 的产生很可能是针藻类鞭毛虫的一个共同特征，我们最近对其他针藻类鞭毛虫（如 Olisthodiscus）的研究结果支持了这一观点。 uteus 和 Fibrocapsa japonica 会产生 O2- 和 H2O2，因为 Chattonella 会产生有害影响或氧自由基。最新研究结果表明，Con A、CBH 和 WGH 等凝集素可刺激 Chattonella 产生 ROS，这表明细胞表面氧化还原酶可能参与 O2 的产生。，这种酶负责凝集素刺激。此外，从黄尾鱼、红鲷鱼和日本比目鱼的皮肤和鳃中制备的鱼粘液刺激了 C.marina 和。 H.akashiwo 以浓度依赖性方式产生更多的 O2-。这些结果表明，鱼粘液增强了 ROS 介导的查托氏菌对鱼的毒性作用。