Induction of Jaw Bone and Tooth Germ from murine embryonic stem cells and human bone marrow stem cells in serum-free defined suspension culture

无血清确定悬浮培养物中鼠胚胎干细胞和人骨髓干细胞诱导颌骨和牙胚

• 批准号: 22659369
• 负责人: OKAMOTO Tetsuji
• 金额: $ 2.07万
• 依托单位: Hiroshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22659369/
• 关键词: マウスES細胞; 骨髄幹細胞; マウスiPS細胞; アクチビン; 軟骨誘導; 無血清培養; ヒトiPS細胞; hESF9無血清培地; 鎖骨頭蓋異栄養症; 歯髄細胞; レトロウイルス; 再生・細胞医療; 創薬スクリーニング; TGFβ; 顎顔面領域

Human Embryonic Stem(hES) cells and human induced Pluripotent Stem(hiPS) cells are commonly maintained on inactivated mouse embryonic fibroblast as feeder cells in medium supplemented with FBS or proprietary replacements. Use of culture media containing undefined or unknown components has limited the development of applications for pluripotent cells because of the relative lack of knowledge regarding cell responses to differentiating growth factors. Therefore we developed a serum-free medium, designated hESF9, in which human ES cells can be maintained in undifferentiated state without feeder cells. We have developed a serum-free defined medium for culturing hiPS cells, which can maintain proliferation, self-renewal and pluripotency. As this simple serum-free adherent monoculture system will allow us to elucidate the cell responses to growth factors under defined conditions, and can eliminate the risk might be brought by undefined pathogens.

人胚胎干 (hES) 细胞和人诱导多能干 (hiPS) 细胞通常作为饲养细胞保存在灭活的小鼠胚胎成纤维细胞上，并添加有 FBS 或专有替代品。由于相对缺乏关于细胞对分化生长因子的反应的知识，使用含有未定义或未知成分的培养基限制了多能细胞的应用开发。因此，我们开发了一种无血清培养基，命名为hESF9，其中人ES细胞可以在没有饲养细胞的情况下维持在未分化状态。我们开发了一种用于培养 hiPS 细胞的无血清限定培养基，可以维持增殖、自我更新和多能性。由于这种简单的无血清贴壁单一培养系统将使我们能够阐明细胞在特定条件下对生长因子的反应，并可以消除不确定病原体可能带来的风险。

项目成果

Growth factor-defined culture medium for human mesenchymal stem cells

用于人间充质干细胞的生长因子限定培养基

• 发表时间: 2011
• 影响因子: 0.7
• 作者: Mimura S; Kimura N; Okamoto; T; et al.
• 通讯作者: et al.

Tissue culture : the unlimited potential

组织培养：无限潜力

• 发表时间: 2010
• 作者: SatoGH; Sato JD; Okamoto T; McKeehan WL; Barnes DW.
• 通讯作者: Barnes DW.

ヒト人工多能性幹(iPS)細胞の単層無血清培養系の確立

人诱导多能干细胞单层无血清培养体系的建立

• 作者: 嶋本顕/木村直大/田原栄俊/岡本哲治

Long term serial cultivation of mouse induced pluripotent stem cells in serum free-and feeder-free growth factor defined culture

在无血清和无饲养层生长因子确定的培养物中长期连续培养小鼠诱导多能干细胞

• 发表时间: 2011
• 作者: Okamoto; T.
• 通讯作者: T.

Growth factor-defined culture medium for human mesenchymal stem cells.

用于人类间充质干细胞的生长因子确定的培养基。

• 发表时间: 2011
• 作者: Mimura S; Kimura N; Okamoto T; et al.
• 通讯作者: et al.