A novel injectable peptide-based hydrogel for myocardial infarction treatment

一种用于心肌梗塞治疗的新型可注射肽基水凝胶

• 批准号: 22659236
• 负责人: NAKATANI Takeshi
• 金额: $ 2.12万
• 依托单位: National Cardiovascular Center Research Institute
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22659236/
• 关键词: 人工臓器学; 心筋梗塞; ゲル注入療法; ペプチドゲル; 組織リモデリング; βターン; 生分解性

Recently, injection of bio-derived hydrogels into myocardial infarction have been investigated for the novel treatment for left ventricular remodeling. In this study, a novel thermoresponsive peptide-type injectable gel matrix composed of naturally occurring sequences were developed, and their therapeutic efficiency was evaluated. A small peptide, Ac-(RVEIKVDI) 2-CONH2 which mimics an anti-parallelβ-sheet region ofβ2-microblobulin, forms hydrogel in situ with high water content in 10v% DMSO aqueous solution. Its solution formed hydrogel in approximately 3 minutes at room temperature. Hydrogelation of the peptide solution is driven by electrostatic and hydrophobic interaction with the conformational change from random coil toβ-sheet structure, as assumed from FT-IR measurement. This hydrogel is rigid(G'> 300 Pa) and is not disrupted in PBS for over 24 hours. In addition, the peptide solution is easily injected into the body by syringe with thin needle, and the resultant hydrogel did not result in serve inflammation. This peptide hydrogel may have implication for in situ forming biomaterials. In addition, we confirmed that the injection of biodegradable hydrogel into the myocardial infarction is as effective as the previous non-degradable one may be via a different mechanism.

最近，人们研究了将生物源水凝胶注射到心肌梗塞中作为左心室重构的新治疗方法。在这项研究中，开发了一种由天然序列组成的新型热响应肽型可注射凝胶基质，并评估了其治疗效果。一种小肽 Ac-(RVEIKVDI) 2-CONH2 模拟 β2-微球蛋白的反平行 β-片层区域，在原位形成水凝胶其溶液在 10v% DMSO 水溶液中在约 3 分钟内形成水凝胶，肽溶液的水凝胶作用是由静电和疏水相互作用驱动的，从无规卷曲到β-折叠结构的构象变化。 -IR 测量。这种水凝胶是刚性的（G'> 300 Pa），在 PBS 中超过 24 小时不会破裂。此外，肽溶液很容易注射到体内。此外，我们证实将可生物降解的水凝胶注射到心肌梗塞中与之前的非生物材料一样有效。 - 可降解的可能是通过一种不同的机制。

项目成果

循環器臨床サピア8心不全の急性期対応

心血管临床 Sapir 8 心力衰竭急性期治疗

• 发表时间: 2010
• 作者: 中谷武嗣

生体親和性ペプチドハイドロゲルの徐放型アンチセンス療法への展開

将生物相容性肽水凝胶开发成缓释反义疗法

• 发表时间: 2011
• 作者: 柿木佐知朗

Mid-to long-term outcomes of cardiovascular tissue replacements utilizing homografts harvested and stored at Japanese Institutional tissue banks

使用在日本机构组织库中收获和储存的同种移植物进行心血管组织替代的中长期结果

• DOI: 10.1007/s00595-010-4459-x
• 发表时间: 2011
• 影响因子: 2.5
• 作者: Kitamura S; Nakatani T; et al.
• 通讯作者: et al.

心臓移植

心脏移植

• 发表时间: 2010
• 作者: 中谷武嗣

Global gene expression analysis of biological responses for porous polymer scaffolds with different molecular architecture

不同分子结构多孔聚合物支架生物反应的全局基因表达分析

• 发表时间: 2011
• 作者: 柿木佐知朗