Regulation of cell functions by deubiquitinating enzymes

通过去泛素化酶调节细胞功能

• 批准号: 22370068
• 负责人: KOMADA Masayuki
• 金额: $ 12.23万
• 依托单位: Tokyo Institute of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22370068/
• 关键词: タンパク質分解; 細胞; タンパク質; 酵素; 細胞生物学; タンパク質品質管理; 変性タンパク質; アグリソーム; ユビキチン; 脱ユビキチン化酵素; プロテアソーム; 核小体; リボソーム生合成

1) Deubiquitinating enzyme USP18 was implicated in the protection of cells from the toxicity of non-native defective proteins by sequestering them to the aggresome in human cells.2) Small molecules that bind to the nucleolar localization signal of deubiquitinating enzyme USP36 were identified and were shown to inhibit the nucleolar localization as well as the function of USP36 in human cells. 3) The ubiquitin-interacting motifs (UIMs) in USP37 were shown to play a role in increasing the isopeptidase activity of USP37 toward ubiquitin chains by binding to the ubiquitin chains and stably positioning them in close proximity to the catalytic core of USP37.

1) 去泛素化酶 USP18 通过将非天然缺陷蛋白隔离在人类细胞的聚集体上，从而保护细胞免受非天然缺陷蛋白的毒性。2) 鉴定并鉴定了与去泛素化酶 USP36 的核仁定位信号结合的小分子。显示可抑制人体细胞中 USP36 的核仁定位和功能。 3) USP37中的泛素相互作用基序(UIM)通过与泛素链结合并将其稳定地定位在紧邻USP37的催化核心的位置，在增加USP37对泛素链的异肽酶活性方面发挥作用。

项目成果

In vivo assembly of the axon initial segment in motor neurons

运动神经元轴突初始段的体内组装

• DOI: 10.1007/s00429-013-0578-7
• 发表时间: 2013
• 作者: Le Bras; B.; Freal; A.; Czarnecki; A.; Legendre; P.; Bullier; E.; Komada; M.; Brophy; P.J.; Davenne; M.;Couraud; F
• 通讯作者: F

Involvement of Src in the membrane skeletal complex, MPP6-4.1G, in Schmidt-Lanterman incisures of mouse myelinated nerve fibers in PNS

Src 参与膜骨骼复合体 MPP6-4.1G，在 PNS 中小鼠有髓神经纤维的施密特-兰特曼切口中

• DOI: 10.1007/s00418-012-1073-6
• 发表时间: 2013
• 作者: Terada; N.; Saitoh; Y.; Ohno; N.; Komada; M.; Yamauchi; J.;Ohno; S.
• 通讯作者: S.

Dominant-negative mutations in α-II spectrin cause West syndrome with severe cerebral hypomyelination, spastic quadriplegia, and developmental delay

α-II 血影蛋白显性失活突变导致 West 综合征，伴有严重脑髓鞘形成不足、痉挛性四肢瘫痪和发育迟缓

• 发表时间: 2010
• 作者: Saitsu; H.
• 通讯作者: H.

The ubiquitin code and its decoding machinery in the endocytic pathway

内吞途径中的泛素代码及其解码机制

• 发表时间: 2013
• 作者: Tanno; H.;Komada; M.
• 通讯作者: M.

Essential function of protein 4.1G in targeting of MPP6 into Schmidt-Lanterman incisures in myelinated nerves

蛋白 4.1G 将 MPP6 靶向有髓神经施密特-兰特曼切口的基本功能

• 发表时间: 2012
• 作者: Terada; N.; Saitoh; Y.; Ohno; N.; Komada; M.; Saitoh; S.; Peles; E.;Ohno; S.
• 通讯作者: S.