マイクロ流体過程を用いた効果的なクルクミン送達のためのポリエステルナノ粒子の合成

使用微流体工艺合成聚酯纳米颗粒以有效递送姜黄素

基本信息

批准号：
17F16720
负责人：
SHEN Amy
金额：
$ 0.7万
依托单位：
Okinawa Institute of Science and Technology Graduate University
依托单位国家：
日本
项目类别：
Grant-in-Aid for JSPS Fellows
财政年份：
2017
资助国家：
日本
起止时间：
2017-04-26 至 2018-03-31
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17F16720/
关键词：
Nanoprecipitation Dynamic light scattering

项目摘要

The proposed research goals are completed smoothly as expected. A novel microfluidic assisted nanoprecipitation strategy that enables synthesis of surfactant-free curcumin encapsulated poly(lactide-co-glycolide) nanoparticles (Cur-PLGA NP) with adjustable particle diameters (30-70 nm) and narrow particle size distribution (polydispersity index less than 0.2). These results confirm that microfluidic devices for are useful in the development of novel polyester nanoparticles for drug delivery. In addition to the physical characterization of the synthesized Cur- PLGA NPs, its degradation kinetics in solution was investigated using UV-vis spectroscopy. The results indicated that these Cur- PLGA NPs are stable for days, furthermore, the encapsulated curcumin molecules are significantly solubilized and stabilized in an aqueous environment. Our Cur-PLGA NP exhibit excellent colloidal stability and inhibit degradation of curcumin. Furthermore, results from in vitro cell studies demonstrate the potential of our Cur-PLGA NP as an effective delivery system for curcumin. Our microfluidic assisted approach offers a controlled and effective nano-biomaterials synthesis of drug delivery system for curcumin, which can be extended to different capsule materials for a variety of biomedical applications. These results have been presented in international symposium and conference and has been published in an international peer-reviewed journal.

拟议的研究目标按预期顺利完成。一种新型的微流体辅助纳米沉淀策略，该策略能够合成无表面活性剂姜黄素封装的聚（乳酸 - 乙二醇 - 乙二醇）纳米颗粒（Cur-Plga NP），可调节的颗粒直径（30-70 nm）（30-70 nm）和狭窄的粒度分布分布（Polydispersity Index Index Index Index bistex）。这些结果证实，用于药物输送的新型聚酯纳米颗粒的开发可用于开发新型聚酯纳米颗粒。除了合成的肠道NP的物理表征外，还使用UV-VIS光谱学研究了其在溶液中的降解动力学。结果表明，这些肠NP稳定了几天，此外，封装的姜黄素分子在水性环境中显着溶解并稳定。我们的Cur-Plga NP表现出极好的胶体稳定性和抑制姜黄素的降解。此外，体外细胞研究的结果证明了我们的cur-plga NP作为姜黄素的有效递送系统的潜力。我们的微流体辅助方法提供了姜黄素药物输送系统的受控且有效的纳米生物材料合成，可以将其扩展到各种生物医学应用的不同胶囊材料。这些结果已在国际研讨会和会议上介绍，并已发表在国际同行评审期刊上。