Effects of GABAergic compounds on the accumbal catecholaminergic activity

GABA能化合物对累积儿茶酚胺能活性的影响

• 批准号: 21792034
• 负责人: UCHIDA Takuya
• 金额: $ 1.58万
• 依托单位: Nihon University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21792034/
• 关键词: GABA; カテコラミン; 大脳基底核; 抗不安薬; 口腔ジスキネジア; SKF38393; 線条体; ドパミン; 脳微小透析法; Ca^<2+>; D_1受容体; ラット; dexamhetamine; reseine; α-methvl-p-tvrosine

The ventral part of striatum called nucleus accumbens is one of the terminal areas of the mesolimbic dopaminergic neurons. It has been shown that the GABA receptor subtype agonists failed to decrease, but its antagonists increased the accumbal dopamine (DA) efflux. Since the GABA synthesis inhibitor enhanced the accumbal DA efflux, but the involvement of accumbal GABA receptor subtypes are unknown. Therefore, we investigated the roles of GABA receptor subtypes in the increase of accumbal DA efflux in freely moving rats by using the in vivo brain microdialysis technique. SKF38393, a benzazepine derivative D_1 receptor agonist is widely used in order to examine the GABA-DA interaction in the striatal structures. SKF38393 is known to increase the striatal DA efflux when applied locally into the dorsal striatum. We analysed the roles of the Ca^<2+> and the intracellular DA pools in the SKF38393-induced striatal DAefflux.

纹状体的腹侧部分称为伏隔核，是中脑边缘多巴胺能神经元的终端区域之一。研究表明，GABA 受体亚型激动剂未能减少，但其拮抗剂却增加了累积多巴胺 (DA) 的流出。由于GABA合成抑制剂增强了accumbal DA流出，但accumbal GABA受体亚型的参与尚不清楚。因此，我们利用体内脑微透析技术研究了GABA受体亚型在自由活动大鼠中积累的DA流出增加中的作用。 SKF38393 是一种苯并氮杂衍生物 D_1 受体激动剂，广泛用于检查纹状体结构中的 GABA-DA 相互作用。已知 SKF38393 局部应用于背侧纹状体时会增加纹状体 DA 流出。我们分析了Ca^2+和细胞内DA池在SKF38393诱导的纹状体DA流出中的作用。

项目成果

Dopamine D_1-like receptors play only a minor role in the increase of striatal dopamine induced by striatally applied SKF38393.

多巴胺 D_1 样受体在纹状体应用 SKF38393 诱导的纹状体多巴胺增加中仅发挥次要作用。

• 发表时间: 2010
• 作者: Sekino R; Saigusa T; Aono Y; Uchida T; Takada K; Oi Y; Koshikawa N; Cools AR.
• 通讯作者: Cools AR.

Dopamine D1-like receptors play only a minor role in the increase of striatal dopamine induced by striatally applied SKF38393

多巴胺 D1 样受体在纹状体应用 SKF38393 诱导的纹状体多巴胺增加中仅发挥次要作用

• 发表时间: 2010
• 影响因子: 5
• 作者: Reiko Sekino

ラット線条体背側部へのSKF38393局所投与で誘発される細胞外DA量の増加におけるCa^の関与

Ca^2+参与SKF38393局部给予大鼠背侧纹状体诱导的细胞外DA量增加

• 发表时间: 2010
• 作者: 関野麗子; 三枝禎; 青野悠里; 内田琢也; 大井良之; 越川憲明
• 通讯作者: 越川憲明