Elucidation of the mechanisms of nociceptor excitation by noxious temperature

阐明有害温度刺激伤害感受器的机制

• 批准号: 21600004
• 负责人: KATANOSAKA Kimiaki
• 金额: $ 3.08万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21600004/
• 关键词: 温度受容; 痛覚; TRPチャネル; 侵害受容器; 一次知覚ニューロン; 温度感覚; 熱; pERK; 痛み; 温度; 感覚; 受容体; 冷痛覚過敏; TRPV2

The mechanisms of heat-and cold-nociception in the nociceptive sensory neurons were examined in rat and mice. No clear correlation between temperature sensitivities of the primary sensory neurons in dorsal root ganglion(DRG) and expression of molecular candidates for nociceptive cold and heat receptors, such as TRPA1 and V2channels, was observed in cultured DRG neurons and anesthetized animals. The data suggested the involvement of other mechanisms independent of these candidate molecules. In addition, I have improved a cold pain assay(cold plate test) to clearly observe the cold pain-related behavior in normal rats and cold hyperalgesia in inflamed rats.

在大鼠和小鼠中检查了伤害性感觉神经元的热和冷伤害性感受机制。在培养的 DRG 神经元和麻醉动物中，没有观察到背根神经节 (DRG) 初级感觉神经元的温度敏感性与伤害性冷热受体候选分子（例如 TRPA1 和 V2 通道）的表达之间存在明显相关性。数据表明独立于这些候选分子的其他机制的参与。此外，我改进了冷痛试验（冷板试验），可以清楚地观察正常大鼠的冷痛相关行为和炎症大鼠的冷痛觉过敏。

项目成果

Sex difference of repeated cold stress(RCS)-induced cutaneous and muscular mechanical hyperalgesia in rats.

重复冷应激（RCS）引起的大鼠皮肤和肌肉机械痛觉过敏的性别差异。

• 发表时间: 2010
• 作者: NASU Teruaki; KATANOSAKA; Kimiaki; MIZUMURA Kazue
• 通讯作者: MIZUMURA Kazue

Excitation and sensitization of nociceptors by bradykinin : What do we know?

缓激肽对伤害感受器的兴奋和敏化：我们知道什么？

• 发表时间: 2009
• 影响因子: 2
• 作者: Mizumura K; Sugiura T; Katanosaka K; Banik RK; Kozaki Y
• 通讯作者: Kozaki Y

The chemical sensitivities and expression of cold-sensitive ion channels in sensory neurons in the persistently inflamed rat

持续性炎症大鼠感觉神经元的化学敏感性和冷敏感离子通道的表达

• 发表时间: 2011
• 作者: Katanosaka K; Ota T; Hirose T; Mori K; Mizumura K
• 通讯作者: Mizumura K

Behavioral hypersensitivity to noxious cold and functional changes of DRG neurons of the rats suffering from persistent joint inflammation

持续性关节炎症大鼠的寒毒行为超敏反应及DRG神经元功能变化

• 发表时间: 2009
• 作者: Katanosaka K; Ota T; Hirose T; Mori K; Mizumura K
• 通讯作者: Mizumura K

The chemical sensitivities and expression of cold-sensitive ionchannels in sensory neurons in the persistently inflamed rat

持续性炎症大鼠感觉神经元的化学敏感性和冷敏感离子通道的表达

• 发表时间: 2011
• 作者: Katanosaka K; Ota T; Hirose T; MoriK; Mizumura K
• 通讯作者: Mizumura K