Analysis of new antigen in anti aquaporin-4 antibody-negative neuromyelitis optica

抗水通道蛋白4抗体阴性视神经脊髓炎新抗原分析

基本信息

批准号：
20790618
负责人：
DOI Hikaru
金额：
$ 2.75万
依托单位：
Kyushu University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Young Scientists (B)
财政年份：
2008
资助国家：
日本
起止时间：
2008 至 2009
项目状态：
已结题

项目摘要

The aim of this study was to find new specific antigen in the central nervous system by researching clinical manifestation and analyzing T cell profile in patients with anti aquaporin-4 (AQP4) antibody-negative neuromyelitis optica (NMO).We first researched the frequency of chronic headaches in patients with neuromyelitis optica and multiple sclerosis (MS). In the study, patients without anti AQP4 antibody had lower frequency of manifestating migraine with aura than patients with anti AQP4 antibody. We secondly researched system of complication. In patients with anti AQP4 antibody-negative optic-spinal MS showed intermediate level of CH50 compared with anti AQP4 antibody-positive NMO and anti AQP4 antibody-negative MS.We further analyzed T helper 1, 17, 2, and 9 cell profile using fresh peripheral blood mononuclear cells (PBMC) and cerebrospinal fluid (CSF) by measuring cytokine such as interferon-gamma, interleukin 17, interleukin 4, and interleukin 9 after the stimulation with PMA and ionomycin.Preliminary results disclosed myelin oligodendrocyte glycoprotein-specific, interferon-gamma producing cells (T helper 1) in a patient with MS. On the other hand, anti AQP4 antibody-negative NMO patients did not show any specific T helper cells so far. We now measuring chemokine receptors such as CXCR3, CCCR6, CCR4, and CCR3 on T helper cells and cytokines mentioned.In summary, we did not find new specific antigen in anti AQP4 antibody-negative NMO. But we showed that anti AQP4 antibody-negative NMO has different characteristics from anti AQP4 antibody-negative MS and anti AQP4 antibody-positive NMO.

本研究的目的是通过研究抗水通道蛋白 4 (AQP4) 抗体阴性视神经脊髓炎 (NMO) 患者的临床表现和 T 细胞谱来寻找中枢神经系统中新的特异性抗原。视神经脊髓炎和多发性硬化症 (MS) 患者的慢性头痛。在该研究中，没有抗AQP4抗体的患者比有抗AQP4抗体的患者表现出先兆偏头痛的频率较低。其次我们研究了并发症的系统。与抗 AQP4 抗体阳性 NMO 和抗 AQP4 抗体阴性 MS 相比，抗 AQP4 抗体阴性视脊髓 MS 患者显示出中等水平的 CH50。我们使用新鲜细胞进一步分析了 T 辅助细胞 1、17、2 和 9 细胞谱。通过测量干扰素-γ、白细胞介素 17、白细胞介素 4 等细胞因子来检测外周血单核细胞 (PBMC) 和脑脊液 (CSF)初步结果显示，多发性硬化症患者体内存在髓磷脂少突胶质细胞糖蛋白特异性、产生干扰素γ的细胞（T辅助细胞1）。另一方面，抗AQP4抗体阴性的NMO患者迄今为止没有表现出任何特异性T辅助细胞。我们现在测量上述T辅助细胞和细胞因子上的CXCR3、CCCR6、CCR4和CCR3等趋化因子受体。综上所述，我们在抗AQP4抗体阴性的NMO中没有发现新的特异性抗原。但我们发现抗AQP4抗体阴性NMO与抗AQP4抗体阴性MS和抗AQP4抗体阳性NMO具有不同的特征。