Analysis for Detection of Immunogenic Site on Aquaporin-4 in Neuromyelitis Optica

视神经脊髓炎Aquaporin-4免疫原位点检测分析

• 批准号: 23591232
• 负责人: MASAHIRO Mori
• 金额: $ 3.24万
• 依托单位: Chiba University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23591232/
• 关键词: 神経免疫疾患; 視神経脊髄炎; 多発性硬化症; 抗アクアポリン４抗体; aquaporin-4; 抗体

We performed anlysis using arrays consisting synthesized 156 peptides using peptide library data from entire human aquaporin-4 (AQP4) 'isoform a' sequences on a cellulose membrane. These peptides were reacted with serum samples obtained from 22 individuals with neuromyelitis optic (NMO), and 8 disease/normal controls. As a result, we can detect the immunogenic site on AQP4 that is recognized by the anti-AQP4 antibody in NMO patients. A peptides derived from an extracellular loop A of AQP4 may be a central immunogenic site on AQP4 in NMO. We have presented these resutls in domestic and international conferences.

我们使用由合成的 156 种肽组成的阵列进行分析，使用来自纤维素膜上整个人水通道蛋白 4 (AQP4)“亚型 a”序列的肽库数据。这些肽与从 22 名视神经脊髓炎 (NMO) 患者和 8 名疾病/正常对照者获得的血清样本发生反应。因此，我们可以检测 NMO 患者中抗 AQP4 抗体识别的 AQP4 上的免疫原性位点。源自AQP4胞外环A的肽可能是NMO中AQP4上的中心免疫原性位点。我们已经在国内和国际会议上展示了这些结果。

项目成果

A case of fulminant neuromyelitis optica presenting with destructive lesions in whole-brain

表现为全脑破坏性病变的暴发性视神经脊髓炎一例

• 发表时间: 2014
• 作者: Sugiyama A; Ito S; Hirano S; Mori M; Kuwabara S
• 通讯作者: Kuwabara S

"Bright spotty lesions" on spinal magnetic resonance imaging differentiate neuromyelitis optica from multiple sclerosis.

脊髓磁共振成像上的“明亮斑点病变”可将视神经脊髓炎与多发性硬化症区分开来。

• DOI: 10.1177/1352458513495581
• 发表时间: 2014
• 作者: Yonezu T; Ito S; Mori M; Ogawa Y; Makino T; Uzawa A; Kuwabara S.
• 通讯作者: Kuwabara S.

Cerebrospinal fluid interleukin-6 and glial fibrillary acidic protein levels are increased during initial neuromyelitis optica attacks

视神经脊髓炎初始发作期间脑脊液白细胞介素 6 和神经胶质纤维酸性蛋白水平升高

• 发表时间: 2013
• 影响因子: 5
• 作者: Uzawa A; Mori M; Sawai S; Masuda S; Muto M; Uchida T; Ito S; Nomura F; Kuwabara S
• 通讯作者: Kuwabara S

Cerebrospinal fluid interleukin-6 and glial fibrillary acidic protein levels are increased during initial neuromyelitis optica attacks.

视神经脊髓炎初次发作时，脑脊液中白细胞介素 6 和神经胶质原纤维酸性蛋白水平升高。

• DOI: 10.1016/j.cca.2013.03.020
• 发表时间: 2013
• 作者: Uzawa A; Mori M; Sawai S; Masuda S; Muto M; Uchida T; Ito S; Nomura F; Kuwabara S.
• 通讯作者: Kuwabara S.

Seroconversion of anti-aquaporin-4 antibody in NMO spectrum disorder : a case report

NMO谱系疾病中抗水通道蛋白4抗体的血清转化：病例报告

• 发表时间: 2012
• 影响因子: 6
• 作者: Mori M; Kawaguchi N; Uzawa A; Nemoto Y; Masuda S; Kuwabara S
• 通讯作者: Kuwabara S