Analysis of lipid-alkyl radical scavenging reactions of vitamin E

维生素E的脂质烷基自由基清除反应分析

• 批准号: 20580125
• 负责人: YAMAUCHI Ryo
• 金额: $ 3.08万
• 依托单位: Gifu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20580125/
• 关键词: ビタミンE; α-トコフェロール; 脂質過酸化; フリーラジカル; トコフェロール; 脂質過酸化反応; フリーラジカル捕捉; 抗酸化剤; 脂質フリーラジカル

Formation of alkyl radicals of unsaturated lipids is the first step of lipid peroxidation. We have studied the lipid-alkyl radical scavenging reactions of vitamin E (α-tocopherol, α-TH). When cholesteryl linoleate (ChL) hydroperoxides were reacted with α-TH at 100℃, two addition products were obtained as 6-O-epoxyChL-α-TH and 6-O-ChL-α-TH. Iron-catalyzed reaction of phosphatidylcholine (PC) hydroperoxides with α-TH under nitrogen gave the addition products, 6-O-epoxyPC-α-TH. A sensitive method using a high-performance liquid chromatography with post-column reduction using platinum as the catalyst followed by fluorescence detection has been developed for the quantification of the addition products of α-TH during the peroxidation of an emulsion of ChL and PC. The addition products of α-TH with alkyl and peroxyl radicals were detected when the emulsion was peroxidized. The results indicate that α-TH may react with lipid-alkyl radicals under oxygen-insufficient conditions.

不饱和脂质烷基自由基的形成是脂质过氧化的第一步，我们研究了胆固醇亚油酸酯（ChL）氢过氧化物与维生素E（α-生育酚，α-TH）反应时的脂质烷基自由基清除反应。在100℃下TH，得到两种加成产物6-O-epoxyChL-α-TH和6-O-ChL-α-TH。在氮气下，磷脂酰胆碱 (PC) 氢过氧化物与 α-TH 发生铁催化反应，生成加成产物 6-O-epoxyPC-α-TH，这是一种采用高效液相色谱法，采用铂作为柱后还原的灵敏方法。催化剂和荧光检测已开发用于定量 ChL 和 PC 乳液过氧化过程中 α-TH 加成产物。乳液过氧化时检测到了烷基和过氧自由基。结果表明，在氧不足的条件下，α-TH 可以与脂质烷基自由基发生反应。

项目成果

抗酸化ビタミンの脂質過酸化抑制機構

抗氧化维生素抑制脂质过氧化的机制

• 发表时间: 2010
• 作者: 山内亮

Pre-existing hydroperoxides play a major role in UVA-induced phospholipid peroxidation

预先存在的氢过氧化物在 UVA 诱导的磷脂过氧化中起主要作用

• 发表时间: 2009
• 作者: Mazari Azzedine; Iwamoto Satoshi; Yamauchi Ryo
• 通讯作者: Yamauchi Ryo

Ultraviolet A-induced peroxidation of phosphatidylcholine in unilamellar liposomes.

紫外线 A 诱导单层脂质体中磷脂酰胆碱的过氧化。

• 发表时间: 2009
• 作者: Mazari; A.; Iwamoto; S.; Yamauchi R.
• 通讯作者: Yamauchi R.

Ultraviolet A-induced peroxidation of phosphatidylcholine in unilamellar liposomes

紫外线 A 诱导单层脂质体中磷脂酰胆碱的过氧化

• 发表时间: 2009
• 作者: Mazari; A.; Iwamoto; S.; Yamauchi; R.
• 通讯作者: R.

Reaction products of γ-tocopherol with (E)-4-oxo-2-nonenal in acidic acetonitrile.

γ-生育酚与 (E)-4-oxo-2-nonenal 在酸性乙腈中的反应产物。

• 发表时间: 2010
• 作者: Saito; F.; Iwamoto; S.; Yamauchi R.
• 通讯作者: Yamauchi R.