Studies on Bilirubin Excretion Mechanism of Fish

鱼类胆红素排泄机制的研究

• 批准号: 63560207
• 负责人: SAKAI Tadashi
• 金额: $ 1.22万
• 依托单位: Miyazaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1989
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63560207/
• 关键词: Bile pigments; High performance liquid chromatography; Bilirubin IX beta; Taurine; Jaundice; In vivo lipid peroxidation; alpha-Tocopherol; Anti-bilirubin monoclonal antibody; ビリルビン; ビリルビン抱合体; 脂質過酸化; 高性能液体クロマトグラフィー; 2-チオバルビツール酸値; α-トコフェロール

1. HPLC method for analysis of bile pigment in fish was constructed. The method is rapid, sensitive, and reliable and seems to be available for measurement of bile pigments in the bile and other tissues of fish.2. Changes in bile pigment contents in the bile of yellowtail fed on taurine with phenylhydrazine induced hemolytic anemia were analyzed by HPLC. The results indicate that taurine plays an important role for bilirubin excretion mechanism of yellowtail.3. TBA values, content of alpha-tocopherol and bile pigments in jaundiced yellowtail were analyzed. The liver and spleen were also observed histologically. The results confirm that the peroxidation of fatty acids in the liver contributes the occurrence of the jaundice.4. Bilirubin-IX beta isomer was detected in the bile of eel, as evidenced by HPLC analysis of free acid and its ethyl anthranilate diazo derivatives. Bilirubin-IX beta accounted for 30-40 % of the total bilirubins in the bile.5. The liver of jaundiced eel was observed histopathologically and enzyme-imunochemically. Bilirubin contents in the plasma were also analyzed by HPLC. The results confirm that the dysfunction of the endocrine systems contributes the occurrence of the disease.

1.建立了鱼类胆色素分析的HPLC方法。该方法快速、灵敏、可靠，可用于鱼类胆汁及其他组织中胆色素的测定。 2．采用HPLC分析饲喂牛磺酸和苯肼引起的溶血性贫血的黄尾鱼胆汁中胆色素含量的变化。结果表明，牛磺酸在黄尾鱼胆红素排泄机制中发挥着重要作用。 3．分析了黄尾鰤鱼的TBA值、α-生育酚和胆汁色素的含量。还对肝脏和脾脏进行组织学观察。结果证实肝脏脂肪酸过氧化是黄疸发生的原因之一。 4．游离酸及其邻氨基苯甲酸乙酯重氮衍生物的 HPLC 分析证明，在鳗鱼的胆汁中检测到胆红素-IX β 异构体。胆红素-IX beta占胆汁中总胆红素的30-40%。5．对黄疸鳗的肝脏进行组织病理学和酶免疫化学观察。还通过HPLC分析血浆中的胆红素含量。结果证实，内分泌系统功能失调导致了该病的发生。

项目成果

Sakai, Tadashi: "Analysis of bile pigments in the bile of yellowtail and eel by high performance liquid chromatography." Agric. Biol. Chem.52. 2967-2968 ((1988))

Sakai，Tadashi：“通过高效液相色谱法分析黄尾鱼和鳗鱼胆汁中的胆汁色素。”

Endo, Makoto: "Pathology of jaundice in the cultured eel Anguilla japonica." J. Fish Disease (1990).

Endo Makoto：“养殖鳗鲡黄疸的病理学。”

Sakai, Tadashi: "Occurrence of bilirubin IX beta in the gallbladder bile of eel, Anguilla japonica." Biochim. Biophys. Acta, 993, 128-130 (1989).

Sakai, Tadashi：“鳗鱼胆囊胆汁中存在胆红素 IX beta。”

Sakai,Tadashi: "Occurrence of bilirubin IXβ in the gallbladder bile of eel,Anguilla japonica." Biochim,Biophys.Acta. 993. 128-130 (1989)

Sakai，Tadashi：“鳗鱼胆囊胆汁中胆红素 IXβ 的存在”，Biochim，Biophys.Acta 993. 128-130 (1989)。

Sakai,Tadashi: "2-Thiobarbituric acid values and contents of α-tocopherol and bile pigments in the liver and muscle of jaundiced yellowtal,Seriola quinqueradiata." Agric.Biol.Chem.53. 1739-1740 (1989)

Sakai，Tadashi：“黄鳝肝脏和肌肉中的 2-硫代巴比妥酸值以及 α-生育酚和胆汁色素的含量。”Agric.Biol.Chem.53（1989）。