The regulation of bone metabolism by amino acid

氨基酸对骨代谢的调节

• 批准号: 23689004
• 负责人: HINOI Eiichi
• 金额: $ 17.47万
• 依托单位: Kanazawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (A)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23689004/
• 关键词: 骨代謝; 骨芽細胞; 破骨細胞; 遺伝子欠損マウス; アミノ酸; 骨粗鬆症

Although amino acid transporters are indispensable for a variety of cellular events in eukaryotic cells, little attention has been paid to their functional role in bone modeling and remodeling so far. Here, we have attempted to demonstrate the possible involvement of a system A amino acid transporter Slc38a1 in the regulation of osteoblastogenesis and osteoclastogenesis in vivo. To that end, cell-specific (osteoblast and osteoclast) knockout mice were generated. In vertebrae of those knockout mice, no marked change was seen in bone volume over tissue volume, osteoblastic parameter and osteoclastic parameter compared with wild-type (WT) mice by bone histomorphometric analyses. Moreover, ovariectomy resulted in a marked reduction of vertebrae bone volume in both WT mice and osteoblast-specific Slc38a1 knockout mice.

尽管氨基酸转运蛋白对于真核细胞中的各种细胞活动是不可或缺的，但迄今为止，它们在骨建模和重塑中的功能作用却很少受到关注。在这里，我们试图证明系统 A 氨基酸转运蛋白 Slc38a1 可能参与体内成骨细胞生成和破骨细胞生成的调节。为此，产生了细胞特异性（成骨细胞和破骨细胞）敲除小鼠。通过骨组织形态学分析，与野生型（WT）小鼠相比，这些基因敲除小鼠的椎骨中骨体积相对于组织体积、成骨细胞参数和破骨细胞参数没有明显变化。此外，卵巢切除导致WT小鼠和成骨细胞特异性Slc38a1敲除小鼠的椎骨体积显着减少。

项目成果

Negative correlation between Per1 and Sox6 expression during chondrogenic differentiation in pre-chondrocytic ATDC5 cells

前软骨细胞 ATDC5 细胞成软骨分化过程中 Per1 和 Sox6 表达呈负相关

• 发表时间: 2013
• 期刊: J. Pharmacol. Sci
• 作者: Le;N.Q.;Binh;N.T.;Takarada;T.;Takarada-Iemata;M.;Hinoi;E. and Yoneda;Y
• 通讯作者: Y

Possible Involvement of Glutamatergic Signaling Machineries in Pathophysiology of Rheumatoid Arthritis

• DOI: 10.1254/jphs.11r03cr
• 发表时间: 2011-07-01
• 期刊: JOURNAL OF PHARMACOLOGICAL SCIENCES
• 影响因子: 3.5
• 作者: Hinoi, Eiichi;Yoneda, Yukio
• 通讯作者: Yoneda, Yukio

Negative correlation between Per1 and Sox6 expression during chondrogenic differentiation in pre-chondrocytic ATDC5 cells.

前软骨细胞 ATDC5 细胞软骨形成分化过程中 Per1 和 Sox6 表达呈负相关。

• 发表时间: 2013
• 期刊: J Pharmacol Sci.
• 作者: Le NQ;Binh NT;Takarada T;Takarada-Iemata M;Hinoi E;Yoneda Y.
• 通讯作者: Yoneda Y.

Growth differentiation factor-15を介した骨細胞 による破骨細胞活性化調節

骨细胞通过生长分化因子 15 调节破骨细胞活化

• 作者: 田坂元一;生沼泉;根岸学;檜井栄一
• 通讯作者: 檜井栄一

低酸素誘導性骨細胞由来因子GDF15による破骨細胞機能調節機構

缺氧诱导骨细胞衍生因子 GDF15 对破骨细胞功能的调节

• 作者: Misu R;Yamamoto K;Yamada A;Noguchi T;Ohno H;Yamamura T;Okamura H;Matsuda F;Ohkura S;Oishi S;Fujii N.;檜井栄一
• 通讯作者: 檜井栄一