Anaerobic spores (C. perfringens) as a possible new fecal source tracking indicator of human fecal pollution.

厌氧孢子（产气荚膜梭菌）作为人类粪便污染的可能的新粪便源跟踪指标。

• 批准号: 23760508
• 负责人: HASHIMOTO Atsushi
• 金额: $ 2.58万
• 依托单位: Prefectural University of Hiroshima
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23760508/
• 关键词: 嫌気性芽胞菌; クリプトスポリジウム; 指標; ウェルシュ菌; 下水; 糞便汚染指標; 毒素遺伝子

Clostridium perfringens survive in various aquatic environments than current traditional fecal indicator. Therefore, C. perfringens considered as suitable indicator for fecal pollution. This research described that the new possibility of C. perfringens as a useful source tracking indicator of human fecal pollution using distributions of cpe positive C. perfringens. To evaluate the efficacy of source tracking indicator of C. perfringens, distributions of C. perfringens spores and there toxin type in sewage and livestock fecal samples were analyzed. C. perfringens spores were isolated from sewage, effluents and fecal samples of cattle and pig by multiplex PCR. Almost all isolates were classified type A. From human sewage and effluents, cpe positive C. perfringens were detected at 30%. Whereas only 1 cattle isolate were positive cpe gene from livestock samples. Distributions of cpe positive C. perfringens were important as a potential source tracking indicator for human fecal pollution.

产气荚膜梭菌比目前传统的粪便指示剂能在各种水生环境中生存。因此，产气荚膜梭菌被认为是粪便污染的合适指标。这项研究描述了利用 cpe 阳性产气荚膜梭菌的分布，将产气荚膜梭菌作为人类粪便污染的有用源跟踪指标的新可能性。为了评估产气荚膜梭菌来源追踪指标的有效性，分析了污水和牲畜粪便样本中产气荚膜梭菌孢子的分布及其毒素类型。通过多重 PCR 从牛和猪的污水、废水和粪便样本中分离出产气荚膜梭菌孢子。几乎所有分离株均被分类为 A 型。从人类污水和废水中，检测到 30% 的 CPE 阳性产气荚膜梭菌。而家畜样本中只有 1 株牛的 cpe 基因呈阳性。 cpe 阳性产气荚膜梭菌的分布作为人类粪便污染的潜在来源追踪指标非常重要。

项目成果

各種水環境から分離した嫌気性芽胞菌の分類

各种水环境中分离的厌氧芽孢菌的分类

• 发表时间: 2011
• 作者: Duan W;He B;Takara K;Luo P;Yamashiki Y;住吉佑介,七種健一郎,河村俊輔,橋本温
• 通讯作者: 住吉佑介,七種健一郎,河村俊輔,橋本温

海域における糞便汚染指標としての嫌気性芽胞菌の有効性

厌氧孢子菌作为海洋区域粪便污染指标的有效性

• 发表时间: 2014
• 作者: 土岡宏章;住吉佑介;原田浩幸;橋本温
• 通讯作者: 橋本温