Non-linear DNA repair responses caused by interference between competitive repair pathways

竞争性修复途径之间的干扰引起的非线性 DNA 修复反应

• 批准号: 23651049
• 负责人: YOKOYA Akinari
• 金额: $ 2.5万
• 依托单位: Japan Atomic Energy Agency
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23651049/
• 关键词: クラスターDNA損傷; DNA修復応答; 酵素反応干渉; 非線形性; 塩基除去修復; Nth; Fpg; 炭素イオンビーム; DNA変性とアニーリング; 放射線、X線、粒子線; 酵素作用機序; DNA変性; 放射線; DNA損傷; 干渉; グリコシレース; プラスミドDNA; DNA2本鎖切断

Ionizing radiation induce localized DNA damage composed of various base lesions (clustered damage). It is expected that damage repair enzymes would be interfered at the localized area on DNA. In this study we aimed to clarify whether the variation of enzymatic processes modify each enzymatic activities at clustered damage site. DNA samples exposed to X-rays were treated with two base excision repair enzymes, namely Nth and Fpg. The enzymes were added sequentially or simultaneously. Contrast to our initial expectation, change of the order of the enzymatic treatments did not affect the activities of the enzymes. This result suggests that, even in a cluster damage site, each base lesions substantially separated not to induce interference between each enzymatic pathways.

电离辐射会引起由各​​种基础损伤组成的局部 DNA 损伤（聚集性损伤）。预计损伤修复酶会在 DNA 的局部区域受到干扰。在本研究中，我们旨在阐明酶过程的变化是否会改变聚集损伤部位的每种酶活性。暴露于 X 射线的 DNA 样本用两种碱基切除修复酶（即 Nth 和 Fpg）进行处理。依次或同时添加酶。与我们最初的预期相反，酶处理顺序的改变并不影响酶的活性。该结果表明，即使在簇损伤部位，每个基底损伤也基本上分开，不会引起每个酶途径之间的干扰。

项目成果

Induction of DNA damage, including abasic sites, in plasmid DNA by carbon ion and X-ray irradiation

通过碳离子和 X 射线照射在质粒 DNA 中诱导 DNA 损伤，包括脱碱基位点

• DOI: 10.1007/s00411-012-0447-4
• 发表时间: 2013
• 期刊: Radiat. Environ. Biophys.
• 作者: Shiina;T.
• 通讯作者: T.

Order effect of repair processes to clustered DNA damage

• DOI: 10.1093/jrr/rrt152
• 发表时间: 2014-03
• 期刊: Journal of Radiation Research
• 影响因子: 2
• 作者: Shiraishi I;Suzuki M;Shikazono N;Fujii K;Yokoya A
• 通讯作者: Yokoya A

クラスターDNA損傷に対する塩基除去修復酵素の作用順序の効果II

碱基切除修复酶的作用顺序对簇DNA损伤的影响II

• 发表时间: 2011
• 作者: 岡本絵梨佳;久道萌;北澤多喜雄;寺岡宏樹;水野直治;古賀公也;志村良治;井上雅子;百瀬邦和;正富宏之;中山翔太;池中良徳;石塚真由美;白石伊世
• 通讯作者: 白石伊世

Chapter 20. Spectroscopic study of radiation-induced DNA lesions and their susceptibility to enzymatic repair. In : Charged particle and photon interactions with matter-recent advances, applications and interfaces, Eds., Y. Hatano, Y. Katsumura, and A. Mo

第 20 章辐射引起的 DNA 损伤及其对酶修复敏感性的光谱研究。

• 发表时间: 2011
• 作者: Yokoya;A.;Fujii;K.;Shikazono;N. and Ukai;M
• 通讯作者: M

クラスターDNA損傷の修復におけるPolIの役割

PolI 在修复簇状 DNA 损伤中的作用

• 发表时间: 2011
• 作者: 新保輝幸;他;大島堅一;鹿園直哉
• 通讯作者: 鹿園直哉