Significance as a therapeutic target and elucidation of EMT in a low oxygen environment of oral squamous cell carcinoma

口腔鳞状细胞癌低氧环境下 EMT 治疗靶点的意义及阐明

• 批准号: 23592968
• 负责人: ISHIDA Takayuki
• 金额: $ 3.16万
• 依托单位: Kagoshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23592968/
• 关键词: 口腔癌; 低酸素; EMT; Notch

We compared invasion and motility and expression of E-cadherin in oral cancer cell linescultured in a normal environment and low oxygen environment. They were increased in a low oxygen environment, but suppressed by the addition of Notch inhibitors. We examined the expression of Notch-related factors in the real time PCR, showed an enhanced expression of Notch signaling in a low-oxygen environment. These results suggested possibility that induce EMT in oral cancer cell lines via the Notch pathway by low oxygen environments, and might suggest Notch passway to be a therapeutic target for inhibiting metastasis of oral cancer.

我们比较了在正常环境和低氧气环境中培养的口腔癌细胞系中E-钙粘着蛋白的入侵和运动和表达。它们在低氧气环境中增加了，但由于添加缺口抑制剂而抑制了它们。我们在实时PCR中检查了Notch相关因子的表达，在低氧环境中显示了Notch信号的表达增强。这些结果表明，低氧环境通过缺口途径在口腔癌细胞系中诱导EMT的可能性，并且可能建议Notch Passway成为抑制口腔癌转移的治疗靶标。

项目成果

The transcription factor Snail enhanced the degradation of E-cadherin and desmoglein 2 in oral squamous cell carcinoma cells

• DOI: 10.1016/j.bbrc.2012.12.060
• 发表时间: 2013-01-18
• 期刊: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
• 影响因子: 3.1
• 作者: Kume, Kenichi;Haraguchi, Misako;Ozawa, Masayuki
• 通讯作者: Ozawa, Masayuki