Investigation of mechanisms of human mast cell activation by respiratory syncytial virus (RSV) and human rhinovirus (HRV) and involvement of asthma inception by these viruses

研究呼吸道合胞病毒（RSV）和人鼻病毒（HRV）激活人类肥大细胞的机制以及这些病毒参与哮喘发作的机制

• 批准号: 23591470
• 负责人: OKAYAMA Yoshimichi
• 金额: $ 3.33万
• 依托单位: Nihon University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23591470/
• 关键词: ヒトマスト細胞; RSウイルス; ヒトライノウイルス; 気管支喘息; 喘息; トリプターゼ; 鼻汁; 喀痰

Although asthma inception is associated with infection with respiratory syncytial virus (RSV) and/or human rhinovirus (HRV), the mechanisms remains unclear. Since we found elevated concentration of mast cell tryptase in nasal discharges/sputa from children under 3 years, who had RSV-induced bronchiolitis with wheezing in the first time in their lives, we hypothesized that these viruses might directly activate human mast cells to induce degranulation. In our in vitro studies, these viruses did not directly activate human mast cells to induce degranulation. Concentrations of RANTES and TGF-beta in nasal secretions/sputa, which were taken at the first time wheezing in their lives from children with no family history of asthma were significantly higher in children who have had wheezing repeatedly than those who have had no repeated wheezing.

尽管哮喘发病与呼吸道合胞病毒（RSV）和/或人鼻病毒（HRV）感染有关，但其机制仍不清楚。由于我们发现3岁以下儿童的鼻分泌物/痰中肥大细胞类胰蛋白酶浓度升高，这些儿童在其一生中第一次患有RSV诱发的细支气管炎并伴有喘息，因此我们假设这些病毒可能直接激活人类肥大细胞以诱导脱颗粒。在我们的体外研究中，这些病毒并没有直接激活人类肥大细胞以诱导脱颗粒。无哮喘家族史儿童首次出现喘息时鼻分泌物/痰中 RANTES 和 TGF-β 的浓度在反复喘息的儿童中显着高于无反复喘息的儿童。

项目成果

Highly expressed cytoplasmic FcεRIβ in human mast cells functions as a negative regulator of the FcRγ-mediated cell activation signal.

人肥大细胞中高表达的细胞质 FcεRIβ 充当 FcRγ 介导的细胞激活信号的负调节因子。

• DOI: 10.1111/cea.12210
• 发表时间: 2014
• 期刊: Clin Exp Allergy
• 影响因子: 6.1
• 作者: Okayama Y;Matsuda A;Kashiwakura JI;Sasaki-Sakamoto T;Nunomura S;Shimokawa T;Yamaguchi K;Takahashi S;Ra C.
• 通讯作者: Ra C.

呼吸器合胞体ウイルスとヒトライノウイルスによるヒト培養マスト細胞脱顆粒に対する影響

呼吸道合胞病毒和人鼻病毒对人培养肥大细胞脱颗粒的影响

• 发表时间: 2013
• 作者: Fujitsuka A;Tsukagoshi H;Arakawa M;Goto-Sugai K;Ryo A;Okayama Y;Mizuta K;Nishina A;Yoshizumi M;Kaburagi Y;Noda M;Tashiro M;Okabe N;Mori M;Yokota S;Kimura H;Okayama Y;岡山吉道,吉住正和,塚越博之,藤田雅弘,坂本朋美,小澤邦壽,羅智靖,木村博一
• 通讯作者: 岡山吉道,吉住正和,塚越博之,藤田雅弘,坂本朋美,小澤邦壽,羅智靖,木村博一

Cellular and humoral immunity of virus-induced asthma.

• DOI: 10.3389/fmicb.2013.00252
• 发表时间: 2013
• 期刊: Frontiers in microbiology
• 影响因子: 5.2
• 作者: Okayama Y

急性呼吸器ウイルス感染と喘息

急性呼吸道病毒感染和哮喘

• 发表时间: 2012
• 期刊: 感染・炎症・免疫
• 作者: Okayama Y;Kashiwakura J;Sasaki-Sakamoto T;Matsumoto K;Hashimoto N;Ohmori K;Kawakami T;Saito H;Ra C;木村博一,梁明秀,岡山吉道
• 通讯作者: 木村博一,梁明秀,岡山吉道

Omalizumab inhibits acceleration of FcεRI-mediated responsiveness of immature human mast cells by IgE

奥马珠单抗通过 IgE 抑制 FcεRI 介导的未成熟人肥大细胞反应性的加速

• 发表时间: 2012
• 期刊: Ann Allergy Asthma Immunol
• 作者: Okayama Y;Kashiwakura J;Sasaki-Sakamoto T;Matsumoto K;Hashimoto N;Ohmori K;Kawakami T;Saito H;Ra C
• 通讯作者: Ra C