Research for the mechanism of aldosterone synthesis in the adrenal gland and the heart.
研究肾上腺和心脏醛固酮合成机制。
基本信息
- 批准号:23591089
- 负责人:
- 金额:$ 3万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:2011
- 资助国家:日本
- 起止时间:2011 至 2013
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
We aimed at the establishment of the experimental system which showed small quantity of aldosterone (Aldo) synthesized in the heart. Various conditions of culture medium and time course were examined using the cultured neonatal rat cardiomyocytes (NRCs). We found that CYP11B2 gene was expressed by short time stimulation of high glucose. This system will be extensively utilizable in the future research of cardiac Aldo synthesis. On the other hand, an unknown action of Aldo was examined by using the Langendorff system. It was found that the pre-treatment of Aldo attenuated ischemia reperfusion injury, suggesting that Aldo per se has a preconditioning action. Also, we newly established the cell culture system which showed the precise intracellular sodium concentration. By this system, we could measure the absolute increase in the intra-cellular sodium concentration stimulated by Aldo and cortisol. This system is also utilizable for the future studies of cardiac hypertrophy and others.
我们的目的是建立显示心脏中合成少量醛固酮(Aldo)的实验系统。使用培养的新生大鼠心肌细胞(NRC)检查各种培养基条件和时间进程。我们发现CYP11B2基因在高糖短时间刺激下表达。该系统将在未来心脏Aldo合成的研究中得到广泛应用。另一方面,使用兰根多夫系统检查了阿尔多的未知动作。研究发现Aldo预处理可减轻缺血再灌注损伤,提示Aldo本身具有预处理作用。此外,我们新建立了细胞培养系统,可显示精确的细胞内钠浓度。通过这个系统,我们可以测量阿尔多和皮质醇刺激的细胞内钠浓度的绝对增加。该系统还可用于心脏肥大等的未来研究。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Aldosterone Preconditioning is Protective during Ischemia-Reperfusion through Modulation of p38 MAPK Signaling in Isolated Rat Heart
醛固酮预处理通过调节 p38 MAPK 信号在离体大鼠心脏缺血再灌注过程中发挥保护作用
- DOI:
- 发表时间:2014
- 期刊:
- 影响因子:0
- 作者:吉野拓哉;吉村道博;他
- 通讯作者:他
A technique for quantifying intracellular free sodium ion using a microplate reader in combination with sodium-binding benzofuran isophthalate and probenecid in cultured neonatal rat cardiomyocytes.
- DOI:10.1186/1756-0500-6-556
- 发表时间:2013-12-26
- 期刊:
- 影响因子:1.8
- 作者:Katoh D;Hongo K;Ito K;Yoshino T;Kayama Y;Komukai K;Kawai M;Date T;Yoshimura M
- 通讯作者:Yoshimura M
Transient decrease in serum potassium level during ischemic attack of acute coronary syndrome: paradoxical contribution of plasma glucose level and glycohemoglobin.
- DOI:10.1186/1475-2840-12-4
- 发表时间:2013-01-04
- 期刊:
- 影响因子:9.3
- 作者:Sekiyama H;Nagoshi T;Komukai K;Matsushima M;Katoh D;Ogawa K;Minai K;Ogawa T;Yoshimura M
- 通讯作者:Yoshimura M
Aldosterone Preconditioning is Protective during Ischemia-Reperfusion through Modulation of p38 MAPK Signaling in Isolated Rat Hearts.
醛固酮预处理通过调节离体大鼠心脏中的 p38 MAPK 信号传导在缺血再灌注过程中发挥保护作用。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:吉野 拓哉;吉村 道博;他
- 通讯作者:他
アルドステロンの合成と作用に関する新しい知見
关于醛固酮合成和作用的新发现
- DOI:
- 发表时间:2014
- 期刊:
- 影响因子:0
- 作者:関 明子;西井 清雅;柴田 洋三郎;小林 靖;萩原 誠久;吉村道博
- 通讯作者:吉村道博
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YOSHIMURA Michihiro其他文献
YOSHIMURA Michihiro的其他文献
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{{ truncateString('YOSHIMURA Michihiro', 18)}}的其他基金
The pathophysiological role of aldosterone in cardiovascular disease.
醛固酮在心血管疾病中的病理生理学作用。
- 批准号:
20590841 - 财政年份:2008
- 资助金额:
$ 3万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Relation between cardiovascular diseases and cardiac hormones
心血管疾病与心脏激素的关系
- 批准号:
17390233 - 财政年份:2005
- 资助金额:
$ 3万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Study of Cardiac Steroidogenesis
心脏类固醇生成的研究
- 批准号:
15390249 - 财政年份:2003
- 资助金额:
$ 3万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Pathosignificance of cardiac aldosterone synthesis in heart failure
心脏醛固酮合成在心力衰竭中的病理意义
- 批准号:
13670729 - 财政年份:2001
- 资助金额:
$ 3万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Molecular biological analysis of coronary spasm
冠状动脉痉挛的分子生物学分析
- 批准号:
11670694 - 财政年份:1999
- 资助金额:
$ 3万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Genetic analysis of endothelial nitric oxide synthase in patients with coronary spastic angina
冠状动脉痉挛性心绞痛患者内皮型一氧化氮合酶基因分析
- 批准号:
09670732 - 财政年份:1997
- 资助金额:
$ 3万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Expression of natriuretic peptides in heart failure
心力衰竭中利尿钠肽的表达
- 批准号:
07670795 - 财政年份:1995
- 资助金额:
$ 3万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
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