Roles of syntaxin1A in the regulation of exocytosis and synaptic vesicle movement in dopaminergic neuron

Syntaxin1A 在多巴胺能神经元胞吐作用和突触小泡运动调节中的作用

• 批准号: 23590307
• 负责人: SASAKAWA Nobuyuki
• 金额: $ 3.41万
• 依托单位: Sophia University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23590307/
• 关键词: exocytosis; chromaffin cell; amperometry; dopaminergic neuron; syntaxin

Chromaffin cells were enzymatically dissociated from adrenal medulla from male C57BL mice. By use of amperometry, single exocytotic events were analyzed in single mouse chromaffin cells with a carbon fiber microelectrode. To characterize a single exocytotic event detected as a single current spike, the spike parameters and the frequency of events were analyzed. To clarify the implication of the interaction of syntaxin 1A and CaMKII in the regulation of exocytosis, we examined the events frequency and the kinetics of single events in the cells prepared from R151G syntaxin 1A knock-in mice. The significant reduction was observed in the frequency of exocytotic events evoked by acetylcholine in knock-in cells. The frequency distribution of the spike height, middle width and area were slightly shifted to smaller values in knock-in cells. Syntaxin 1A knock-in seemed to affect not only the event frequency but also the kinetics of the fusion process.

嗜铬细胞通过酶法从雄性 C57BL 小鼠的肾上腺髓质中分离出来。通过使用电流分析法，使用碳纤维微电极分析单个小鼠嗜铬细胞中的单个胞吐事件。为了表征检测为单个电流尖峰的单个胞吐事件，分析了尖峰参数和事件频率。为了阐明突触蛋白 1A 和 CaMKII 相互作用在胞吐作用调节中的含义，我们检查了 R151G 突触蛋白 1A 敲入小鼠制备的细胞中的事件频率和单个事件的动力学。在敲入细胞中乙酰胆碱引起的胞吐事件的频率显着降低。在敲入细胞中，尖峰高度、中间宽度和面积的频率分布稍微偏移到较小的值。 Syntaxin 1A 敲入似乎不仅影响事件频率，还影响融合过程的动力学。

项目成果

Effects of D/Cmosaic botulinum neurotoxin on the regulation of exocytosis in single adrenal chromaffin cells

D/Cmosaic肉毒神经毒素对单个肾上腺嗜铬细胞胞吐作用调节的影响

• 发表时间: 2012
• 作者: Hayashi、M.;Shibata;Y.;Nakamura;K.;KOzaki;S.and Sasakawa;N.
• 通讯作者: N.

Dissociation of inositol polyphosphates from the C2B domain of synaptotagmine facilitates spontaneous release of catecholamines in adrenal chromaffin cells. A suggestive evidence of a fusion clamp by synaptotagmin.

肌醇多磷酸从突触他明的 C2B 结构域解离有助于肾上腺嗜铬细胞中儿茶酚胺的自发释放。

• 发表时间: 2011
• 期刊: Neuropharmacology
• 影响因子: 4.7
• 作者: Sasakawa;N.;Ohara-Imaizumi;M.;Fukuda;M.;Kobayama;H.;Kumakura;K
• 通讯作者: K

Unique biological activity of botulinum D/C mosaic neurotoxin

肉毒杆菌D/C嵌合神经毒素独特的生物活性

• 发表时间: 2012
• 作者: 中村佳司;幸田知子;柴田優斗;塚本健太郎;有満秀幸;向本雅郁;笹川展幸;小崎俊司
• 通讯作者: 小崎俊司

Effects of D/C mosaic botulinum neurotoxin on the regulation of exocytosis in single adrenal chromaffin cells

D/C嵌合肉毒神经毒素对单个肾上腺嗜铬细胞胞吐作用调节的影响

• 发表时间: 2012
• 作者: 林光紀;柴田優斗;中村佳司;小崎俊司;笹川展幸
• 通讯作者: 笹川展幸

Involvement of theα1D-adrenergic receptor in methamphetamine-induced hyperthermia and neurotoxicity in rats

α1D-肾上腺素能受体参与甲基苯丙胺诱导的大鼠高热和神经毒性

• 发表时间: 2013
• 期刊: Neurotox Res
• 影响因子: 3.7
• 作者: Kikuchi-Utumi;K;Ishizaka;M.;Matsumura;N.;Watanabe;M.;Aoyama;K.;Sasakawa;N.;Nakaki;T.
• 通讯作者: T.