Therapeutic ultrasound for treatment of hypothyroidism

治疗性超声治疗甲状腺功能减退症

• 批准号: 10551996
• 负责人: Vesna Zderic
• 金额: $ 8.08万
• 依托单位: GEORGE WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-02-01 至 2024-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10551996
• 关键词: Adipocytes; Adrenal Glands; Affect; Apoptotic; Area; Biological Assay; Biological Sciences; Biomedical Engineering; Body Temperature; Body Weight; Breathing; CASP3 gene; Calcitonin; Catecholamines; Cattle; Cell Survival; Cell physiology; Cells; Chromaffin Cells; Clinical; Defect; Deficiency Diseases; Disease; Dose; Dyes; Elderly; Endocrine; Endocrine Glands; Endocrine System Diseases; Endocrine system; Endocrinologist; Endocrinology; Engineering; Enzyme-Linked Immunosorbent Assay; Event; Exclusion; Fluorescence; Fluorescence Microscopy; Generations; Goals; Heart Rate; Hormonal; Hormone replacement therapy; Hormones; Human; Hydrogen Peroxide; Hypothyroidism; In Vitro; Insulin; Measures; Medicine; Menstrual cycle; Metabolic Diseases; Metabolic hormone; Metabolism; Methods; Modeling; Modification; Molecular; Non-Insulin-Dependent Diabetes Mellitus; Organ; Pancreas; Pathology; Pathway interactions; Persons; Physiological; Population; Process; Publishing; Research; Research Personnel; Rodent; Role; Safety; Schools; Structure of beta Cell of islet; Structure of thyroid parafollicular cell; Testing; Therapeutic; Therapeutic Effect; Thyroid Gland; Thyroid Hormones; Thyrotropin-Releasing Hormone; Thyroxine; Time; Tissues; Triiodothyronine; Trypan Blue; Ultrasonic Therapy; United States; Universities; Washington; Woman; Work; adiponectin; combat; cytotoxic; effectiveness evaluation; effectiveness testing; human old age (65+); improved; in vitro Model; in vivo; interest; medical schools; men; novel; obese person; older patient; pharmacologic; programs; sodium ion; therapeutic target; treatment optimization; ultrasound

Project Abstract The thyroid gland is a regulatory organ within the endocrine system vital to many essential processes including breathing and heart rate, metabolism and body weight, menstrual cycles, and body temperature. Hypothyroidism is a common disease especially among the elderly, affecting 5–20% of women and 3–8% of men, and is on the rise in the United States. Thyroid hormone replacement therapy is used for treatment of hypothyroidism but suffers from various problems with therapy optimization and dose adjustment. Our main goal is to determine whether ultrasound can serve as a non-invasive and non-pharmacological method for treatment of hypothyroidism. Here, we propose a novel exploratory study to determine if and how ultrasound may stimulate and control thyroid gland function such as synthesis and release of metabolic hormones triiodothyronine (T3) and thyroxine (T4) in a physiologically relevant context. Our previous and ongoing research work has shown that low intensity therapeutic ultrasound releases insulin from pancreatic beta cells and rodent pancreas at physiological levels without compromising cell viability. This finding opens the doors for possible use of ultrasound therapy in ameliorating secretory defects in other endocrine diseases. The long-term goal of our collaborative research program among Schools of Engineering, Biological Sciences and Medicine at The George Washington University is to develop a mechanistic understanding of the role of therapeutic ultrasound in enhancing release of hormones as a potential treatment of various endocrine diseases. The main objective of this proposal is to determine whether and in what way ultrasound may impact release of thyroid hormones in an in vitro rodent thyroid gland model. Specific Aim 1 of this research work will focus on determining the effectiveness and safety of ultrasound stimulation of the thyroid gland for release of metabolic hormones with the hypothesis that ultrasound treatment promotes release of endocrine thyroid hormones T3 and T3 with little or no other secretory products being released. Specific Aim 2 will focus on determination of mechanisms of ultrasound impact on release of thyroid metabolic hormones with the hypothesis that the mechanism of ultrasound action on the release of thyroid metabolic hormones T3 and T4 is related to the transport of sodium ions and generation of hydrogen peroxide. If shown successful, our proposed approach may open new strategies to combat various endocrine and metabolic diseases.

项目摘要 甲状腺是内分泌系统中对许多基本过程至关重要的内分泌系统中的调节器官 呼吸和心率，代谢和体重，月经周期和体温。甲状腺功能减退症 是一种常见疾病，尤其是在老年人中，影响了5-20％的女性和3-8％的男性，并且在 在美国上升。甲状腺替代疗法用于治疗甲状腺功能减退症，但 在治疗优化和调整剂量调整方面遭受了各种问题。我们的主要目标是确定 超声是否可以用作一种无创和非药理学方法来治疗 甲状腺功能减退症。在这里，我们提出了一项新的探索性研究，以确定超声是否以及如何超声 刺激和控制甲状腺功能，例如合成和代谢恐怖的释放 在物理相关的情况下，三碘甲状腺素（T3）和甲状腺素（T4）。我们以前的和正在进行的 研究工作表明，低强度热超声从胰腺β细胞中释放胰岛素 和啮齿动物胰腺的物理水平，没有损害细胞活力。这一发现为 可能在其他内分泌疾病中使用超声疗法来改善分泌缺陷。长期 我们在工程，生物科学和医学学院之间的合作研究计划的目标 乔治华盛顿大学将对治疗作用有一种机械理解 超声在增强激素的释放中，作为各种内分泌疾病的潜在治疗方法。主 该建议的目的是确定超声是否以及以何种方式影响甲状腺的释放 体外啮齿动物甲状腺模型中的骑马。这项研究工作的特定目的1将重点放在确定 甲状腺超声刺激对释放代谢激素的有效性和安全性 超声处理促进内分泌甲状腺激素T3和T3的假设很少 或没有其他分泌产品。具体目标2将重点放在确定机制 超声对甲状腺代谢训练的释放的影响，假设是 超声动作对甲状腺代谢激素T3和T4的释放与钠的运输有关 离子和过氧化氢的产生。如果显示成功，我们提出的方法可能会开放新的策略 对抗各种内分泌和代谢疾病。