Prevention of atherosclerosis by apple procyanidins and elucidation of their mechanism

苹果原花青素预防动脉粥样硬化及其机制的阐明

• 批准号: 23500996
• 负责人: SHOJI Toshihiko
• 金额: $ 2.91万
• 依托单位: National Agriculture and Food Research Organization
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23500996/
• 关键词: リンゴ; プロシアニジン類; 動脈硬化; ApoE欠損マウス; RAW264.7細胞; 動脈硬化予防; apple; procyanidins; procyanidin; 3T3-L1 cell; RAW246.7 cell

We investigated the preventive effect of procyanidins, which is major phytochemicals in apple, on atherosclerosis in ApoE-deficient mice and 3T3-L1 cells. After the ingestion of high fat diet for 20 weeks, the body weight and mesenteric, perirenal, and testis fat weights in the high fat diet group significantly increased than those of normal diet group. And, thrombus-like structure was observed in vessel of mice of the high fat diet group. On the contrary, the increases of body weight and mesenteric, perirenal, and testis fat weights in the apple procyanidin group significantly inhibited than those of the high fat diet group. Furthermore, thrombus-like structure was observed in vessel of mice of the high fat diet group. Furthermore, APC prevented the exprresion of Aim gene in the ApoE-deficient mice and the secretion of TNF-a in RAW264.7 cells.

我们研究了苹果中的主要植物化学物质原花青素对 ApoE 缺陷小鼠和 3T3-L1 细胞的动脉粥样硬化的预防作用。摄入高脂饮食20周后，高脂饮食组的体重以及肠系膜、肾周、睾丸脂肪重量较正常饮食组显着增加。并且，在高脂肪饮食组的小鼠血管中观察到血栓样结构。相反，苹果原花青素组体重和肠系膜、肾周、睾丸脂肪重量的增加较高脂饮食组显着受到抑制。此外，在高脂肪饮食组的小鼠血管中观察到血栓样结构。此外，APC 阻止 ApoE 缺陷小鼠中 Aim 基因的表达以及 RAW264.7 细胞中 TNF-a 的分泌。