Molecular mechanisms of alveolar epithelial healing process in acute lung injury

急性肺损伤肺泡上皮愈合过程的分子机制

基本信息

批准号：
23390376
负责人：
SUGAHARA Kazuhiro
金额：
$ 12.15万
依托单位：
University of the Ryukyus
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2011
资助国家：
日本
起止时间：
2011-04-01 至 2014-03-31
项目状态：
已结题

项目摘要

1. We have demonstrated that 1) keratinocyte growth factor (KGF) is a potent factor for proliferation and differentiation of alveolar type II cells, 2) intratracheal instillation of KGF prevented acute lung injury by bleomycin and HCl. 2. Ethyl pyruvate reportedly has anti-inflammatory actions through inhibition of NF-kB. We confirmed that treatment of cultured alveolar epithelial cells with tumor necrosis factor a (TNFa) increased the NF-kB/DNA-binding activity, which was strongly inhibited with incubating with ethyl pyruvate. We also demonstrated that ethyl pyruvate interacts with RelA and p50 to inhibit their functions at multiple points. 3. Legionella pneumophila has flagella, causes pulmonary fibrosis. Microarray analysis in cultured alveolar epithelial cells indicated that flagellin increased fibronectin level and reduced E-cadherin level, which suggested that flagellin cooperates with TGF-b and induces EMT in alveolar epithelial cells, and causes fibrosis.

1. 我们已经证明 1) 角质形成细胞生长因子 (KGF) 是 II 型肺泡细胞增殖和分化的有效因子，2) 气管内滴注 KGF 可预防博来霉素和 HCl 造成的急性肺损伤。 2. 据报道，丙酮酸乙酯通过抑制 NF-kB 具有抗炎作用。我们证实，用肿瘤坏死因子 a (TNFa) 处理培养的肺泡上皮细胞会增加 NF-kB/DNA 结合活性，而与丙酮酸乙酯一起孵育会强烈抑制这种活性。我们还证明丙酮酸乙酯与 RelA 和 p50 相互作用，在多个点抑制它们的功能。 3.嗜肺军团菌有鞭毛，引起肺纤维化。培养肺泡上皮细胞的微阵列分析表明，鞭毛蛋白增加纤连蛋白水平，降低E-钙粘蛋白水平，这表明鞭毛蛋白与TGF-b协同诱导肺泡上皮细胞发生EMT，导致纤维化。

项目成果

期刊论文数量（0）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Long-term treatment with San'o-shashin-to, Kampo medicine, markedly ameliorates cardiac ischemia-reperfusion injury in ovariectomized rats via the Redox-dependent mechanism

汉方药Sano-shashin-to的长期治疗通过氧化还原依赖性机制显着改善卵巢切除大鼠的心脏缺血再灌注损伤

DOI：
发表时间：
2013
期刊：
Circulation Journal
影响因子：
3.3
作者：
Mayuko Sakanashi;T. Matsuzaki;K. Noguchi;J. Nakasone;M. Sakanashi;T. Uchida;M. Kina-Tanada;H. Kubita;K. Arakaki;A. Tanimoto;N. Yanagihara;M. Sakanashi;Y. Ohya;H. Masuzaki;S. Ishiuchi;K. Sugahara;M. Tsutsui
通讯作者：
M. Tsutsui

Availability of initial distribution volume of glucose (IDVG) for the volume control in pulmonary decompression illness; "The chokes"

葡萄糖初始分布容积 (IDVG) 用于肺减压疾病容量控制的可用性；