Involvement of neurotrophic factors in muscular pain and mechanisms for their production and action in the muscle

神经营养因子参与肌肉疼痛及其在肌肉中的产生和作用机制

• 批准号: 23390154
• 负责人: MIZUMURA Kazue
• 金额: $ 11.98万
• 依托单位: Chubu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23390154/
• 关键词: 筋性疼痛; 機械痛覚過敏; 神経成長因子; 筋細径線維受容器; グリア細胞由来神経栄養因子; 遅発性筋痛; ATP; EP2受容体; グリア由来神経栄養因子; ｐERK; 感作; TRPV1; TRPV4; 神経損傷; 虚血

This study was planned to clarify involvement of neurotrophic factors in muscular pain models, their action mechanisms on muscular nociceptors, and factors that were involved in production of neurotrophic factors. Delayed onset muscle soreness (DOMS) induced after lengthening contraction (LC) was used for a model of muscle pain. We showed that EP2 receptor was involved in the COX-2-GDNF (glial cell derived neurotrophic factor) pathway leading to DOMS, and that upregulation of NGF and GDNF was induced in the muscle cells/satellite cells after LC. We also showed effects of NGF and GDNF on mechanically activated currents in cultured dorsal root ganglion neurons, sensitizing effect of GDNF on muscular A-delta mechano-sensitive nociceptors, and collaboration of NGF and GDNF in inducing muscular mechanical hyperalgesia.

该研究计划阐明神经营养因素参与肌肉疼痛模型，其对肌肉伤害感受器的作用机制以及与神经营养因子产生有关的因素。延长收缩（LC）引起的延迟发作肌肉酸痛（DOMS）用于肌肉疼痛模型。我们表明，EP2受体参与导致DOMS的COX-2-GDNF（神经胶质细胞衍生的神经营养因子）途径，并且LC后LC后肌肉细胞/卫星细胞中诱导NGF和GDNF的上调。我们还显示了NGF和GDNF对培养的背根神经元中机械激活电流的影响，GDNF对肌肉A-DELTA机械敏感的伤害感受器的敏化作用以及NGF和GDNF在诱导肌肉机械性高速度诱导的NGF和GDNF的协作中。

项目成果

Muscular mechanical hyperalgesia occurs in a stretch speed-dependent manner after lengthening contraction in rats

大鼠延长收缩后，肌肉机械痛觉过敏以拉伸速度依赖性方式发生

• 发表时间: 2013
• 作者: Hayashi K;Mori T;Agata N;Abe M;Mizumura K;Yamanaka A;Kawakami K;Taguchi T.
• 通讯作者: Taguchi T.

遅発性筋痛は伸張性収縮の伸張関節範囲と角速度に依存して生じる

延迟性肌肉酸痛的发生取决于伸展关节范围和伸展收缩的角速度

• 发表时间: 2013
• 作者: Fotiadis D;Kanai Y;Palacin M;林功栄，阿部真博，水村和枝，山中章弘，田口徹．
• 通讯作者: 林功栄，阿部真博，水村和枝，山中章弘，田口徹．

ATP release from the rat skeletal muscle differs among muscle contraction forms

大鼠骨骼肌释放的 ATP 因肌肉收缩形式而异

• 发表时间: 2014
• 期刊: Jounral of Physiological Sciences
• 作者: 井川達弘;阿部義人;白石充典;津田 誠,井上和秀;植田正;Murase,S.; Mizumura,K.
• 通讯作者: Murase,S.; Mizumura,K.

各種筋痛モデルにおける筋機械痛覚過敏と神経栄養因子の検討

各种肌肉疼痛模型中肌肉机械性痛觉过敏和神经营养因子的检查

• 作者: 山中健三;圓藤吟史;荻野景規;那須民江;水村和枝
• 通讯作者: 水村和枝

Repeated intramuscular injections of nerve growth factor induced progressive muscle hyperalgesia, facilitated temproral summation and expanded pain areas

重复肌内注射神经生长因子可诱导进行性肌肉痛觉过敏，促进颞叶总和并扩大疼痛区域

• 发表时间: 2013
• 期刊: Pain
• 影响因子: 7.4
• 作者: Hayashi K;Shiozawa S;Ozaki N;Mizumura K;and Graven-Nielsen T
• 通讯作者: and Graven-Nielsen T