Molecular analysis of skin cell activation by orally administered functional food substances

口服功能性食品物质激活皮肤细胞的分子分析

• 批准号: 23380072
• 负责人: SHIMIZU Makoto
• 金额: $ 12.23万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23380072/
• 关键词: 皮膚; セラミド; コラーゲン; 遺伝子発現; 繊維芽細胞; 角化細胞; 共培養; スフィンゴシン; DNAマイクロアレイ

Gene expression changes induced in damaged mice skin by Mg-deficient diet or UV irradiation were elucidated by DNA microarray and PCR analyses. Oral administration of ECM components, such as ceramide and collagen, was found to attenuate the gene expression changes. In vitro experiments using a fibroblast-keratinocyte co-culture system constructed with mouse primary skin cells or human skin cell lines showed that metabolites of ceramide as well as collagen peptides induced similar effects on gene expression of skin cells. These results suggest that ECM components are digested and/or metabolized after oral administration, reach the skin tissue, and then affect keratinocyte functions via modulating fibroblast-keratinocyte interactions.

DNA 微阵列和 PCR 分析阐明了缺镁饮食或紫外线照射在受损小鼠皮肤中诱导的基因表达变化。研究发现，口服神经酰胺和胶原蛋白等 ECM 成分可以减弱基因表达的变化。使用小鼠原代皮肤细胞或人皮肤细胞系构建的成纤维细胞-角质形成细胞共培养系统进行的体外实验表明，神经酰胺和胶原蛋白肽的代谢物对皮肤细胞的基因表达产生类似的影响。这些结果表明，ECM成分在口服后被消化和/或代谢，到达皮肤组织，然后通过调节成纤维细胞-角质形成细胞相互作用来影响角质形成细胞功能。

项目成果

共培養系においてセラミドが皮膚細胞に及ぼす影響

神经酰胺对共培养系统中皮肤细胞的影响

• 发表时间: 2014
• 作者: 赤木 裕;レ ヴ ラン フォン;薩 秀夫;戸塚 護;千田和広;清水 誠
• 通讯作者: 清水 誠

Organotypic coculture of mouse skin cells as a model for studying the effects of collagen peptide

小鼠皮肤细胞的器官型共培养作为研究胶原蛋白肽作用的模型

• 发表时间: 2013
• 作者: Phuong;L.V.;Takatori. R.;Iwamoto,T.;Sato;K.;Totsuka,M.;Satsu;H.;Chida,K.;Shimizu;M
• 通讯作者: M

Effects of prolyl-hydroxyproline, a collagen peptide, on gene expression of mouse skin cells in coculture

胶原肽脯氨酰羟脯氨酸对共培养小鼠皮肤细胞基因表达的影响

• 发表时间: 2012
• 作者: Phuong;L.V.;Takatori. R.;Iwamoto,T.;Sato;K.;Totsuka,M.;Satsu;H.;Chida,K.;Shimizu;M
• 通讯作者: M

Effects of prolyl-hydroxyproline, a collagen peptide, on gene expression of mouse skin cells in co-culture

胶原肽脯氨酰羟脯氨酸对共培养小鼠皮肤细胞基因表达的影响

• 发表时间: 2012
• 作者: Phuong;L.V
• 通讯作者: L.V

Effects of Oral Administration of Glucosylceramide on Gene Expression Changes in Hairless Mouse Skin: Comparison of Whole Skin, Epidermis, and Dermis

口服葡萄糖神经酰胺对无毛小鼠皮肤基因表达变化的影响：整个皮肤、表皮和真皮的比较

• DOI: 10.1271/bbb.130318
• 发表时间: 2013
• 期刊: Bioscience, Biotechnology, and Biochemistry
• 作者: Takatori;R.;Lan Phuong;L.V.;Iwamoto;T.;Satsu;H.;Totsuka;M.;Shimizu;M.
• 通讯作者: M.