Research on Full-protocol Disease Marker Screening System using Integrated Lab-on-a-CD for Immunoassay

使用集成Lab-on-a-CD进行免疫分析的全方案疾病标志物筛查系统的研究

• 批准号: 23350035
• 负责人: UTSUMI Yuichi
• 金额: $ 11.98万
• 依托单位: University of Hyogo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23350035/
• 关键词: チップ分析; 生体分析; 免疫反応; バイオリアクター; ELISA; マイクロ化学システム; Lab-on-a-chip; Lab-on–a-chip

This paper reports a new type of lab-on-a-CD device with three-dimensional microchannel networks and vertical capillary bundle structure. The device consist of the multiple lab-on-a-CD chips with conventional planer microchannels and vertical microchannels which constructed on the disks made of poly-dimethylsiloxane (PDMS) and poly-methylmethacrilrate (PMMA). The PMMA layer with vertical channel were fabricated by deep X-ray lithography using synchrotron radiation. We designed and fabricated the chips for aim of taking enzyme-linked immunosorbent assay(ELISA). Three-dimensional liquid transportation through Lab-on-a-CD structure is suggested by computational fluid dynamics and it is successfully demonstrated chips. Detection of immunogroblin G(IgG) from mouse is successfully demonstrated in the three-dimensional devices and it is notable that the result suggests quite high-sensitive implying detection limit down to 1ng/mL by primitive result.

本文报道了一种新型的 CD 实验室设备，具有三维微通道网络和垂直毛细管束结构。该装置由多个 CD 实验室芯片组成，这些芯片具有传统的平面微通道和垂直微通道，构建在由聚二甲基硅氧烷 (PDMS) 和聚甲基丙烯酸甲酯 (PMMA) 制成的圆盘上。具有垂直通道的PMMA层是通过使用同步加速器辐射的深X射线光刻技术制造的。我们设计并制造了用于酶联免疫吸附测定（ELISA）的芯片。计算流体动力学提出了通过Lab-on-a-CD结构进行三维液体传输，并成功在芯片上进行了演示。在三维装置中成功演示了小鼠免疫球蛋白 G(IgG) 的检测，值得注意的是，结果表明灵敏度相当高，这意味着原始结果的检测限低至 1ng/mL。

项目成果

Development of Bio-Microsystems and Sensors using nano/micro-structures

使用纳米/微米结构开发生物微系统和传感器

• 发表时间: 2012
• 作者: 山崎悟;坂部輝御;長谷川健;泰永愛佳;Yuichi Utsumi
• 通讯作者: Yuichi Utsumi

High-sensitive Enzyme-linked Immunosorbent Assey in Three-dimensional Lab-on-a-CD

三维 CD 实验室中的高灵敏度酶联免疫吸附测定

• 发表时间: 2011
• 作者: Hiroki Nose;Tsukasa Azeta;Yoshiaki Ukita;Saki Kondo;Chiwa Kataoka;Yuichi Utsumi
• 通讯作者: Yuichi Utsumi

Three Dimensional Gold Nanostructure for Plasmonic Sensing

用于等离子体传感的三维金纳米结构

• 发表时间: 2012
• 作者: Ryo Takahashi;Takao Fukuoka;Yuichi Utsumi
• 通讯作者: Yuichi Utsumi

Three-Dimensional Magneto Impedance Sensor using a Multi Layers Electroplating technique

采用多层电镀技术的三维磁阻抗传感器

• 发表时间: 2013
• 作者: Yuji Yoritama;S. Yamamoto;T. Uchiyama ;Akinobu Yamaguchi;Yuichi Utsumi
• 通讯作者: Yuichi Utsumi

Contamination-Free Internally-Triggered Automatic Flow Sequencing for Microfluidics

适用于微流体的无污染内部触发自动流程排序

• 发表时间: 2012
• 作者: Masaki Ishizawa;Yuichi Utsumi
• 通讯作者: Yuichi Utsumi