Research on high sensitive handy ELISA system for multi-environmental analysis using 3D micro fluidic networks

利用3D微流体网络进行多环境分析的高灵敏度便携式ELISA系统的研究

• 批准号: 20510015
• 负责人: UTSUMI Yuichi
• 金额: $ 2.91万
• 依托单位: University of Hyogo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20510015/
• 关键词: 環境分析; チップ分析; 生体分析; マイクロメカトロニクス; バイオリアクター; 反応流

There have been paid much attention to the lab-on-a-CD for the use of enazyme-linked immunosorbent assay (ELISA), due to its convenience for the automation of multiple microreactors [1-2]. However it is difficult to simultaneously realize the performance and integration of multiple reactor units due to the limited chip area. One of the biggest problem arise from the limitation is inadequate sensitivity due to shortage of surface area for the immobilization of antibody. we propose a new type lab-on-a-CD concept based on three-dimensional (3D) microchannel network. This paper reports high-sensitive detection of poly-chlorinatedbiphenyl (PCB) by using bundle-like capillary structure on a 3D lab-on-a-CD.

酶联免疫吸附测定（ELISA）的 CD 实验室因其方便的多个微反应器的自动化而受到广泛关注 [1-2]。然而，由于芯片面积有限，很难同时实现多个反应器单元的性能和集成。该限制产生的最大问题之一是由于固定抗体的表面积不足而导致灵敏度不足。我们提出了一种基于三维（3D）微通道网络的新型 CD 实验室概念。本文报道了在 3D Lab-on-a-CD 上使用束状毛细管结构对多氯联苯 (PCB) 进行高灵敏度检测。

项目成果

Enzyme-Linked Immunosorbent Assay using Antibody Bound Fluoroplastic Microstructure

使用抗体结合的氟塑料微结构进行酶联免疫吸附测定

• 发表时间: 2009
• 作者: Y.Ukita;S.Kondo;T.Azeta;C.Kataoka;M.Takeo;S.Negoro;Y.Utsumi
• 通讯作者: Y.Utsumi

Fabrication of Polytetrafluoroethylene microparts by High-Energy X-ray Induced Etching

高能 X 射线诱导蚀刻制备聚四氟乙烯微型零件

• 发表时间: 2008
• 期刊: Japanese Journal of Applied Physics 47
• 作者: Yoshiaki Ukita;Mitsuyoshi Kishihara;Yuichi Haruyama;Kazuhiro Kanda;Shinji Matsui;Kozo Michiji;Yuichi Utsumi
• 通讯作者: Yuichi Utsumi

High Efficiency Mixing by the Use of Cross-Linked Micro Capillary Fluid Filter

使用交联微毛细管流体过滤器进行高效混合

• 发表时间: 2008
• 期刊: Microsystem Technologies 14
• 作者: Kuniyo Fujiwara;Yoshiaki Ukita;Masahiro Takeo;Seiji Negoro;Tomohiko Kanie;MakotoKatayama;Yuichi Utsumi
• 通讯作者: Yuichi Utsumi

High-Sensitive Enzyme-linked immunosorbent assay in Three-dimensional LAB-ON-A-CD

三维 LAB-ON-A-CD 中的高灵敏度酶联免疫吸附测定

• 发表时间: 2010
• 作者: Tsukasa Azeta;Yoshiaki Ukita;Yoshito Hirose;Saki Kondo;Yuichi Utsumi
• 通讯作者: Yuichi Utsumi

Vertical Liquid Transportation Through Capillary Bundle Structure Using Centrifugal Force

利用离心力通过毛细管束结构垂直输送液体

• 发表时间: 2009
• 作者: Saki Kondo;Tsukasa Azeta;Yoshiaki Ukita;Yuichi Utsumi
• 通讯作者: Yuichi Utsumi