Development of the general tags for formation of hetero-complex
用于形成异质复合物的通用标签的开发
基本信息
- 批准号:22657028
- 负责人:
- 金额:$ 2.25万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Challenging Exploratory Research
- 财政年份:2010
- 资助国家:日本
- 起止时间:2010 至 2011
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
In order to develop the general tags, HA-tags which can control and promote the formation of a hetero complex, we first searched candidate peptides of the tag from the protein crystal structures(more than the 3A resolution) from PDB in silico. Finally two kinds of hetero-dimer tag(2HA-tag) were selected. We constructed model proteins with 2HA-tag fusion at the N or C terminal, and over-expressed them in Escherichia coli. The experiments of hetero-dimer formation are in progress.
为了开发能够控制和促进异源复合物形成的通用标签HA标签,我们首先从PDB in silico的蛋白质晶体结构(超过3A分辨率)中搜索标签的候选肽。最后选择了两种异源二聚体标签(2HA-tag)。我们构建了 N 端或 C 端带有 2HA 标签融合的模型蛋白,并在大肠杆菌中过表达它们。异源二聚体形成的实验正在进行中。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Crystallization and preliminary X-ray crystallographic analysis of eIF5BΔN and the eIF5BΔN-eIF1AΔN complex
eIF5BΔN 和 eIF5BΔN-eIF1AΔN 复合物的结晶和初步 X 射线晶体学分析
- DOI:10.1107/s1744309111015910
- 发表时间:2011
- 期刊:
- 影响因子:0
- 作者:Aiping Zheng;Reo Yamamoto;Masaaki Sokabe;Isao Tanaka and Min Yao
- 通讯作者:Isao Tanaka and Min Yao
Preliminary X-ray crystallographic study of the receptor-binding domain of the D/C mosaic neurotoxin from Clostridium botulinum
肉毒杆菌 D/C 嵌合神经毒素受体结合域的 X 射线晶体学初步研究
- DOI:
- 发表时间:2010
- 期刊:
- 影响因子:0
- 作者:Nuemket N.;Tanaka Y.;Tsukamoto K.;Tsuji T.;Nakamura K.;Kozaki S.;Yao M.;Tanaka I.
- 通讯作者:Tanaka I.
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YAO Min其他文献
Chinese Herbal Medicine for Osteosarcoma in the mouse:A Systematic Review
中草药治疗小鼠骨肉瘤的系统评价
- DOI:
- 发表时间:
2019 - 期刊:
- 影响因子:2.9
- 作者:
CHANG Jun-li;WANG Wen-yi;LI Yi-mian;HU Shao-pu;YAO Min;CUI Xue-jun;ZHI Wen-lan;SHI Qi;WANG Yong-jun;YANG Yan-ping - 通讯作者:
YANG Yan-ping
YAO Min的其他文献
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{{ truncateString('YAO Min', 18)}}的其他基金
Realization of eukaryotic synthesis speed in Escherichia coli by cassette exchange of ribosome stalk complex
通过核糖体茎复合物盒交换实现大肠杆菌中真核合成速度
- 批准号:
26650013 - 财政年份:2014
- 资助金额:
$ 2.25万 - 项目类别:
Grant-in-Aid for Challenging Exploratory Research
Explanation of assembly mechanism of 5S RNP in eukaryotic ribosome biosynthesis
真核核糖体生物合成中5S RNP组装机制的解释
- 批准号:
25291008 - 财政年份:2013
- 资助金额:
$ 2.25万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
A novel method to crystallizing proteins by using two-dimensional organic-inorganichybrid material
一种利用二维有机-无机杂化材料结晶蛋白质的新方法
- 批准号:
24657068 - 财政年份:2012
- 资助金额:
$ 2.25万 - 项目类别:
Grant-in-Aid for Challenging Exploratory Research
The study on the multi-molecular reaction mechanism of transamidosome for Gln-tRNA(Gln) synthesis
转酰胺体合成Gln-tRNA(Gln)的多分子反应机制研究
- 批准号:
21370041 - 财政年份:2009
- 资助金额:
$ 2.25万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
The coupling mechanism of glutaminase and kinase in GatCAB
GatCAB中谷氨酰胺酶和激酶的偶联机制
- 批准号:
19370037 - 财政年份:2007
- 资助金额:
$ 2.25万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Structure analysis of multi-factor complex of eukaryotic translation initiation factor
真核翻译起始因子多因子复合物的结构分析
- 批准号:
16570089 - 财政年份:2004
- 资助金额:
$ 2.25万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Analysis reaction mechanism of ACC deaminase by X-ray crystallography
X射线晶体学分析ACC脱氨酶反应机理
- 批准号:
11680600 - 财政年份:1999
- 资助金额:
$ 2.25万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
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