Inhibitory effect of androgen on the constitutive gene expression of hepatic drug metabolizing enzymes and its mechanism

雄激素对肝脏药物代谢酶组成型基因表达的抑制作用及其机制

• 批准号: 22590166
• 负责人: KOJIMA Misaki
• 金额: $ 2.83万
• 依托单位: National Institute of Agrobiological Sciences
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22590166/
• 关键词: アンドロゲン; 薬物代謝酵素; 肝臓; CYP; 性差; 系統差; 優性遺伝; ブタ; 第II相薬物代謝酵素; 構成的発現; IL-1; Cyp7a1; PGC-1α; アンドロゲン濃度; 常染色体性優性遺伝; 転写因子; CYP1A1; CYP1A2; 品種間差

Sex differences in the constitutive expression of hepatic drug metabolizing enzymes, including cytochrome P4501A (CYP1A) subfamily enzymes, in the pig liver have been examined using 5-month-old Meishan, Landrace, and their F1 cross breeds, LM and ML pigs. The levels of serum androgen in all the pigs used were also examined, and the relationship between the serum androgen levels and the expression levels of hepatic metabolizing enzymes, which were observed to be expressed in a sex-dependent manner, were investigated. All the results indicated that serum andorogen down-regulated the constitutive gene expression of several drug metabolizing enzymes including CYP1As with threshold. Furthermore, it was demonstrated for the first time that serum androgen level in male pigs was determined by autosomal dominat inheritance.

在猪肝脏中，已经使用5个月大的Meishan，Landrace及其F1交叉品种，LM和ML猪在猪肝脏中检查了肝药物代谢酶的性别差异，包括细胞色素P4501A（CYP1A）亚家族酶。还检查了所有使用的猪中血清雄激素的水平，并研究了血清雄激素水平与肝代谢酶的表达水平之间的关系，观察到以性依赖性方式表达。所有结果表明，血清雄激素下调了几种药物代谢酶的组成型基因表达，包括阈值的CYP1A。此外，首次证明了雄性猪中的血清雄激素水平是由常染色体统治遗传确定的。

项目成果

Sex-differences in the constitutive gene expression levels of CYPs in the pig liver

猪肝中CYPs组成型基因表达水平的性别差异

• 发表时间: 2012
• 作者: Misaki Kojima;Masashi Sekimoto;Masakuni Degawa
• 通讯作者: Masakuni Degawa

Androgen-mediated down-regulation of CYP1A subfamily genes in the pig liver

• DOI: 10.1677/joe-10-0160
• 发表时间: 2010-11-01
• 期刊: JOURNAL OF ENDOCRINOLOGY
• 影响因子: 4
• 作者: Kojima, Misaki;Sekimoto, Masashi;Degawa, Masakuni
• 通讯作者: Degawa, Masakuni

Sex-differences in the constitutive gene expression levels of CYPs in the pig liver: Androgen-mediated Dwonregulation

猪肝脏 CYP 组成型基因表达水平的性别差异：雄激素介导的 Dwon 调节

• 作者: M. Koima;M. Sekimoto and M. Degawa
• 通讯作者: M. Sekimoto and M. Degawa

Androgn-mediated down-regulation of CYP1A subfamily genes in the pig liver

雄激素介导的猪肝脏 CYP1A 亚家族基因下调

• 发表时间: 2010
• 期刊: J. Endocrinol
• 作者: M. Kojima;M. Sekimoto and M. Degawa
• 通讯作者: M. Sekimoto and M. Degawa

マウス肝でのcholetserol 7α-hydroxylaseおよびその転写因子の構成的遺伝子発現におけるInterleulin-1の寄与

Interleulin-1对小鼠肝脏胆固醇7α-羟化酶及其转录因子组成型基因表达的贡献

• 作者: 小島美咲;芦野隆;吉田武美;岩倉洋一郎;関本征史;出川雅邦
• 通讯作者: 出川雅邦