Molecular epidemiological and cytological studies on Plasmodium falciparum distributing in Southeast Asian continent or island areas

东南亚大陆及岛屿地区恶性疟原虫的分子流行病学和细胞学研究

基本信息

批准号：
22406011
负责人：
KAWAMOTO Fumihiko
金额：
$ 9.9万
依托单位：
Oita University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2010
资助国家：
日本
起止时间：
2010 至 2012
项目状态：
已结题

项目摘要

Our previous findings showed that wild isolates of Plasmodium falciparum collected from Myanmar and Vietnam were easily adapted to in vitro culture, whereas those collected from Indonesian islands failed to be adapted. These results may suggest that Indonesian wild isolates has a tendency not to easily adapt to in vitro culture, differing from those distributing in continental SEA. Therefore, this study was conducted for confirmation of these previous findings and for cytological and molecular epidemiological comparisons between wild isolates collected from continental SEA or from Indonesian islands. All of 20 wild isolates collected from many Indonesian islands could not be adapted to in vitro culture again; all parasites disappeared within 7 days in culture. On the other hand, seven wild isolates collected from Vietnam and Cambodia were adapted to in vitro culture. Particularly, an isolate collected from Vietnam grew well, and it formed many young gametocytes. This isolates could be induced mass-production of gametocytes by decreasing glucose level in the culture medium. By using this isolate and an Indonesian isolate, comparison at molecular level was tried by the Roche Genome Sequencer FLX+ System (the Pyrosequence method), but any difference between them was not found yet.

我们之前的研究结果表明，从缅甸和越南收集的恶性疟原虫野生分离株很容易适应体外培养，而从印度尼西亚岛屿收集的恶性疟原虫则无法适应。这些结果可能表明，与分布在东南亚大陆的野生分离株不同，印度尼西亚野生分离株具有不易适应体外培养的倾向。因此，进行这项研究是为了证实这些先前的发现，并对从大陆东南亚或印度尼西亚岛屿收集的野生分离株进行细胞学和分子流行病学比较。从印尼多个岛屿采集的20株野生分离株全部无法再次适应体外培养；所有寄生虫在培养 7 天内消失。另一方面，从越南和柬埔寨收集的七个野生分离株适应了体外培养。特别是从越南收集的分离物生长良好，并且形成了许多年轻的配子体。该分离株可以通过降低培养基中的葡萄糖水平来诱导配子体的大量生产。通过使用该分离株和印度尼西亚分离株，通过罗氏基因组测序仪FLX+系统（焦磷酸测序法）尝试在分子水平上进行比较，但尚未发现它们之间有任何差异。

项目成果

期刊论文数量（0）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Single-nucleotide polymorphism, linkage disequilibrium and geographic structure in the malaria parasite Plasmodium vivax: prospects for genome-wide association studies.

DOI：
10.1186/1471-2156-11-65
发表时间：
2010-07-13
期刊：
BMC genetics
影响因子：
2.9
作者：
Orjuela-Sánchez P;Karunaweera ND;da Silva-Nunes M;da Silva NS;Scopel KK;Gonçalves RM;Amaratunga C;Sá JM;Socheat D;Fairhust RM;Gunawardena S;Thavakodirasah T;Galapaththy GL;Abeysinghe R;Kawamoto F;Wirth DF;Ferreira MU
通讯作者：
Ferreira MU

Genotypes of Candida albicans involved in development of candidiasis and their distribution in oral cavity of non-candidiasis individuals

与念珠菌病发生相关的白色念珠菌基因型及其在非念珠菌病个体口腔中的分布

DOI：
发表时间：
2011
期刊：
Med. Mycol. J.
影响因子：
0
作者：
Y. Takagi;H. Hattori;H. Adachi;S. Takakura;T. Horii;A. Chindamporn;H. Kitai;R. Tanaka;T. Yaguchi;H. Fukano;F. Kawamoto;K. Shimozato;T. Kanbe
通讯作者：
T. Kanbe

「東南アジアにおけるマラリアの分子疫学的研究」に至った長い道のり

“东南亚疟疾分子流行病学研究”的漫长历程