Gene analysis and in vitro culture of the new human malaria parasites

新型人类疟原虫的基因分析和体外培养

• 批准号: 14570213
• 负责人: KAWAMOTO Fumihiko
• 金额: $ 2.18万
• 依托单位: OITA UNIVERSITY (FACULTY OF MEDICINE) (2003)Nagoya University (2002)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14570213/
• 关键词: New human malaria parasite; New Plasmodium malariae; New Plasmodium ovale; Gene analysis; SSUrRNA; Cysteine protease; Cytochrome B; In vitro culture; オーキネート表面抗原; Plasmodium minuta様原虫; 繁殖周期

Using wild isolates of the new types of P. malariae and P. ovale collected from Myanmar and Indonesia, SSUrRNA, cytochrome b, cysteine protease or ookinete surface antigen genes were analyzed. In addition, in vitro culture was examined using wild isolates.(1)The new type of P. ovale possessed three different sequences of SSUrRNA gene, and all of them were differed from two sequences of the classic type, Nigerian-I.(2)The new types of P malariae and P. ovale had different sequences of cysteine protease gene from each classic type, Uganda-1 or Nigerian-I.(3)The new P ovale had different sequences of ookinete surface antigen genes from Nigerian-I.(4)In analysis of cytochrome b gene, the new P. ovale had a different sequence from the Harding strain, whereas the new P. malariae were also different from Uganda-1, but there was no difference between the two new types.(5)In vitro cultures of these new human malaria parasites were performed, using an enriched medium with glucose and 20% human serum. After cultivation for 24-48 hrs, they grew to young schizont stages, but further growth was not observed.

利用从缅甸和印度尼西亚采集的新型三日疟和卵形疟原虫的野生分离株，对SSUrRNA、细胞色素b、半胱氨酸蛋白酶或动合蛋白表面抗原基因进行了分析。此外，还利用野生分离株进行了体外培养检测。(1)新型卵圆孢子虫具有3个不同的SSUrRNA基因序列，均与经典型尼日利亚-I的两个序列不同。(2 )新的疟原虫和卵形疟原虫与经典型乌干达-1或尼日利亚-I的半胱氨酸蛋白酶基因序列不同。(3)新的卵形疟原虫与经典型疟原虫P. ovale的半胱氨酸蛋白酶基因序列不同。 (4)在细胞色素b基因分析中，新的卵形疟原虫与Harding株有不同的序列，而新的三日疟原虫也与Uganda-1不同，但有两种新类型之间没有差异。(5)使用含有葡萄糖和20%人血清的富集培养基对这些新的人类疟原虫进行了体外培养。培养24-48小时后，它们生长到幼裂殖体阶段，但没有观察到进一步的生长。

项目成果

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坦图拉，I.S.

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Safitri, I. 等人：“天然恶性疟原虫种群中疟疾疫苗候选丝氨酸重复抗原 (SERA) 氨基末端区域的序列多样性”Parasitol.Int.. 117-131 (2003)。

Win, T.T.et al.: "Sequence analysis of nuclear and mitochondrial genes of the human malara parasite Plasmodium ovale supports its division into at least two variants."Emerg.Inf.Dis.. 10(印刷中). (2004)

Win, T.T. 等人：“对人类疟原虫卵形疟原虫的核和线粒体基因的序列分析支持其至少分为两种变体。”Emerg.Inf.Dis.10（印刷中）。

win, T.T.et al.: "Sequence analysis of nuclear and mitochondrial genes of the human malaria parasite Plasmodium ovale supports its division into at least two variants"Emerg.Inf.Dis.. (印刷中).

win, T.T. 等人：“对人类疟疾寄生虫卵形疟原虫的核和线粒体基因的序列分析支持其分为至少两个变体”Emerg.Inf.Dis..（出版中）。

Tantular, I.S., Kawamoto, F.: "An improved, simple screening method for detection of glucose-6-phosphate dehydrogenase deficiency"Trop.Med.Int.Health. 8・6. 569-574 (2003)

Tantular，I.S.，Kawamoto，F.：“一种改进的、简单的检测葡萄糖-6-磷酸脱氢酶缺陷的方法”Trop.Med.Int.Health 8・6（2003）。