Molecular epidemiology on two new human malaria in Southeast Asia

东南亚两种新人类疟疾的分子流行病学

基本信息

批准号：
13576006
负责人：
KAWAMOTO Fumihiko
金额：
$ 6.21万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2002
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13576006/
关键词：
international collaboration Plasmodium malariae Plasmodium ovale malaria gene analysis SSUrRNA gene field survey Myaynmar : Indonesia : Vietnam : Thailand 新型卵型マラリア原虫野外調査ミャンマー:インドネシア:タイ分子疫学的調査ミャンマー:インドネシア:ベトナム

项目摘要

During 2001 to 2002, field surveys of malaria were conducted in Southeast Asia, including Myanmar, Indonesia, Vietnam and Thailand, by using a rapid diagnosis with acridine orange staining, and gene analysis was performed on Plasmodium malariae and P.ovale isolates.1. In Myanmar, malaria surveys were conducted at many villages in Sagain Division, Rakhine State, Mon State, Mandalay Division, Shan State and Tanintharyi Division during 2001 to 2002. In all villages, we detected many patients infected with the new types of P.malariae and several patients infected with P.ovale. In addition, single infections with the new types of P.malariae and P.ovale were also detected from many villages.2. In Indonesia, we surveyed malaria at Maumere and Lalantuka, Flores island, East Nusa Tenggara Province, during 2001 to 2002. At both places, many patients infected with P.malariae and some patients infected with P.ovale were detected.3. In Vietnam, malaria survey was done at Bao Loc, Lam Dong Province, on September 2001, but the new types of P.malariae and P.ovale were not found.4. In Thailand, malaria surveys were performed at Sangkhlaburi on November, 2002, and three cases infected with the new types of P.malariae were detected. But, P.ovale infection was not detected.5. The SSUrRNA gene was analyzed in all wild isolates of P.malariae and P.ovale collected from Southeast Asia, and all, except for one P.ovale isolate, were reconfirmed as the new variant types. In addition, single infections with the new types of P.malariae were also reconfirmed by molecular diagnosis.6. Analysis of the full SSUrRNA gene in variant P.ovale isolates collected from Southeast Asia indicated that they were actually the new type.

2001年至2002年，利用吖啶橙染色快速诊断技术，在缅甸、印度尼西亚、越南、泰国等东南亚地区进行了疟疾现场调查，并对三日疟原虫和卵形疟原虫进行了基因分析。 1．在缅甸，2001年至2002年期间，在萨盖省、若开邦、孟邦、曼德勒省、掸邦和德林达依省的许多村庄进行了疟疾调查。在所有村庄，我们都发现了许多感染新型疟原虫的患者。以及数名感染 P.ovale 的患者。此外，多个村庄还发现了新型三日疟和卵形疟原虫单一感染病例。2．在印度尼西亚，我们于2001年至2002年对东努沙登加拉省弗洛勒斯岛毛梅雷和拉兰图卡的疟疾进行了调查。在这两个地方，都发现了许多感染疟原虫的患者和一些感染卵形疟原虫的患者。 3．越南2001年9月在林同省保禄县进行了疟疾调查，但未发现新的三日疟和卵形疟原虫。 4． 2002年11月，泰国在Sangkhlaburi进行了疟疾调查，发现了三例新型疟原虫感染病例。但未检测到P.ovale感染。5．对从东南亚收集的所有疟原虫和卵形疟原虫野生分离株的 SSUrRNA 基因进行了分析，除一种卵形疟原虫分离株外，所有分离株均被重新确认为新的变种类型。此外，分子诊断也再次证实了新型疟原虫的单次感染。6．对从东南亚收集的卵形 P.ovale 变体分离株的完整 SSUrRNA 基因进行分析表明，它们实际上是新类型。