Research for novel diagnostic marker of gynecologic tumors and establishment of rapid and low invasive detection system by super-targeting antibody.

妇科肿瘤新型诊断标志物研究及超靶向抗体快速低创检测体系的建立。

• 批准号: 22390118
• 负责人: KATO Kazunori
• 金额: $ 11.23万
• 依托单位: Toyo University (2011-2012)Juntendo University (2010)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22390118/
• 关键词: 癌; 腫瘍マーカー; 抗体; 診断薬; 婦人科腫瘍; 卵巣癌; 子宮体癌; EpCAM; TROP2; 癌幹細胞; エクソソーム; ビーズアレイ

We could succeed to generate the target antibody against the gynecologic cancers and to establish a high-sensitive detection system of novel tumor marker. The first, we generated mAb to human NCAM2 expressed on hormone-dependent prostate and breast cancer. In addition, we established seven mAbs against ovarian cancer and one to breast cancer. The second, in attempt to improve diagnosis for gynecologic cancers, we developed new sandwich ELISA and immunochromatography systems detecting human sEpCAM, sCD147, sEphA2 and sTROP2, respectively, with high sensitivity and specificity. The third, we examined the biomarker candidates that were predominantly expressed in cancer stem cells of endometrial cancers. Thus, this approach is feasible for non-invasive and high sensitive diagnosis for gynecologic cancers.

我们可以成功地生成针对妇科癌症的靶抗体，并建立一种新型肿瘤标记的高敏性检测系统。首先，我们对依赖激素依赖性前列腺和乳腺癌表达的人类NCAM2产生了mAb。此外，我们建立了七个针对卵巢癌的mab，一个mab对着乳腺癌。第二，为了改善妇科癌症的诊断，我们开发了新的三明治ELISA和免疫色谱系统，分别以高灵敏度和特异性检测人类Sepcam，SCD147，Sepha2和Strop2。第三，我们检查了主要在子宫内膜癌的癌症干细胞中表达的生物标志物候选物。因此，这种方法对于妇科癌症的非侵入性和高敏感诊断是可行的。

项目成果

Butyrate inhibitis the Self-Renewal Capacity of Endometrial Tumor Side-Population Cells by Induction a DNA Damage Response.

丁酸盐通过诱导 DNA 损伤反应来抑制子宫内膜肿瘤侧群细胞的自我更新能力。

• 发表时间: 2011
• 期刊: Mol Cancer Ther.
• 作者: Kato K;Kuhara A;Yoneda T;Inoue T;Takao T;Ohgami T;Dan L;Kuboyama A;Kusunoki S;Takeda S;Wake N.Sodium
• 通讯作者: Wake N.Sodium

Effect of transient TCDD exposure on immortalized human trophoblast-derived cell lines

• DOI: 10.1177/0960327111424305
• 发表时间: 2012-06-01
• 期刊: HUMAN & EXPERIMENTAL TOXICOLOGY
• 影响因子: 2.8
• 作者: Fukushima, K.;Tsukimori, K.;Wake, N.
• 通讯作者: Wake, N.

Stemness property of the human esophageal squamous cell carcinoma in three-dimensional culture.

三维培养人食管鳞状细胞癌的干细胞特性。

• 发表时间: 2012
• 作者: 藤原大介; 加藤和則 ;野原茂夫;松原久 昭;岩沼佳美;梶山美明
• 通讯作者: 梶山美明

Epigenetic modulation enhances therapeutic effect of anti-IL13R alpha 2 antibody in human mesothelioma xenografts

表观遗传调节增强抗 IL13R α2 抗体在人间皮瘤异种移植物中的治疗效果

• 发表时间: 2010
• 作者: Shioda N;Beppu H;Fukuda T;Li E;Kitajima I;Fukunaga K;竹之内信
• 通讯作者: 竹之内信

Neural cell adhesion molecule 2 as a target molecule for prostate and breast cancer gene therapy

• DOI: 10.1111/j.1349-7006.2011.01855.x
• 发表时间: 2011-04
• 期刊: Cancer Science
• 影响因子: 5.7
• 作者: Shu Takahashi;Kazunori Kato;Kiminori Nakamura;R. Nakano;K. Kubota;H. Hamada