Novel approach to the distribution and function of globin proteins in aquatic organisms

水生生物中珠蛋白分布和功能的新方法

• 批准号: 22380115
• 负责人: OCHIAI Yoshihiro
• 金额: $ 12.31万
• 依托单位: Tokai University (2011-2012)The University of Tokyo (2010)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22380115/
• 关键词: グロビン類; タンパク質; 構造; 機能; 分布; グロビン; 生理機能; ミオグロビン; 一次構造; 立体構造; 分子進化; 系統解析; 組換え体; 構造安定性; 分子動力学

Attempts were made to clone the genes coding globin proteins (especially myoglobin) from a variety of fish species, and based on the deduced amino acid sequences and bioinformatic analyses, the profiles of molecular evolution and environment adaptation were examined. Distribution of globins was investigated by measuring the transcription and expression levels of globin genes in various tissues of several fish species. The properties and tissue distribution of mammalian globins were also examined. On the other hand, the physiological roles of myoglobin other than oxygen molecule binding were also found.

试图从各种鱼类中克隆编码球蛋白蛋白（尤其是肌红蛋白）的基因，并根据推导的氨基酸序列和生物信息学分析，检查了分子进化和环境适应的曲线。通过测量几种鱼类的各种组织中球蛋白基因的转录和表达水平来研究球蛋白的分布。还检查了哺乳动物全球素的特性和组织分布。另一方面，还发现了除氧分子结合以外的肌红蛋白的生理作用。

项目成果

Fast preparation and autooxidation profile of tuna myoglobin

金枪鱼肌红蛋白的快速制备和自氧化特性

• 发表时间: 2012
• 作者: Mala Nurilmala;Shugo Watabe;Yoshihiro Ochiai
• 通讯作者: Yoshihiro Ochiai

Fast preparation and autoxidation profile of tuna myoglobin

金枪鱼肌红蛋白的快速制备和自氧化特性

• 发表时间: 2012
• 作者: M. Nurilmala;S. Watabe;Y. Ochiai
• 通讯作者: Y. Ochiai

Structural characterization of piscine globin superfamily proteins

鱼珠蛋白超家族蛋白的结构表征

• 发表时间: 2012
• 期刊: World Acad. Sci. Eng. Technol
• 作者: Kodani S;Bicz J;Song L;Deeth RJ;Ohnishi-kameyama M;Yoshida M;Ochi K;Challis GL;Y. Ochiai
• 通讯作者: Y. Ochiai

Temperature-dependent structural perturbation of tuna myoglobin

金枪鱼肌红蛋白的温度依赖性结构扰动

• 发表时间: 2011
• 期刊: World Acad.Sci.Eng.Technol
• 作者: C.A. Strussmann;T. Oikawa and T. Otake;西川淳;Y.Ochiai
• 通讯作者: Y.Ochiai

Thermal Denaturation Profiles of Tuna Myoglobin

• DOI: 10.1271/bbb.100290
• 发表时间: 2010-08
• 期刊: Bioscience, Biotechnology, and Biochemistry
• 作者: Y. Ochiai;Yoshiaki Watanabe;Hideo Ozawa;S. Ikegami;N. Uchida;S. Watabe