Exhaustive studies on filament forming proteins of molluskan muscle

对软体动物肌肉丝形成蛋白的详尽研究

• 批准号: 19380119
• 负责人: OCHIAI Yoshihiro
• 金额: $ 13.06万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19380119/
• 关键词: 軟体類; 筋肉; タンパク質; フィラメント; 構造; 軟体動物; フィラメント形成; 一次構造; 立体構造; ミオシン; フィラメント形成能; 構造機能解析

Muscular filament-forming proteins, namely, myosin heavy chain, actin and tropomyosin from four species of mollusks (cephalopods) have been investigated. As for tropomyosin, those from other species were examined for their structural stability. Primary structure analysis based on cDNA cloning, phylogenetic analysis based on amino acid sequence, and functional and structural analysis based on tertiary structure simulation have revealed the specificity and uniqueness of those contractile proteins. The results obtained suggested that mollusks, especially cephalopods, have evolved protein related to muscle contraction to compensate for their specialized locomotion activities. On the other hand, the properties of myosin heavy chain, etc. would give important information for the effective utilization and processing of mollusks.

已经研究了来自四种软体动物（头足类）的肌球蛋白重链，肌动蛋白和肌球蛋白的肌肉丝形成蛋白。至于tropomyosin，研究了其他物种的结构稳定性。基于cDNA克隆，基于氨基酸序列的系统发育分析以及基于三级结构仿真的功能和结构分析的一级结构分析已揭示了这些收缩蛋白的特异性和独特性。获得的结果表明，软体动物，尤其是头足类动物已经进化了与肌肉收缩有关的蛋白质，以补偿其专门的运动活性。另一方面，肌球蛋白重链的特性等将提供重要的信息，以有效地利用和处理软体动物。

项目成果

軟体動物筋肉アクチンの構造上の特徴

软体动物肌动蛋白的结构特征

• 发表时间: 2009
• 作者: 落合芳博;王国峰;渡部終五
• 通讯作者: 渡部終五

Role of skip residues in myosin rod as revealed by synthetic peptides.

合成肽揭示了肌球蛋白杆中跳跃残基的作用。

• 发表时间: 2008
• 作者: Y. Ochiai;G.F. Wang;S. Watabe
• 通讯作者: S. Watabe

cDNA cloning and deduced primary structure of common octopus Octopus vulgaris muscle myosin heavy chain

普通章鱼Octopus vulgaris肌肉肌球蛋白重链的cDNA克隆及一级结构推导

• 发表时间: 2008
• 作者: Guofeng Wang;Shugo Watabe;Yoshihiro Ochiai
• 通讯作者: Yoshihiro Ochiai

Characterization of subfragment-2 regions of myosins from invertebrate and vertebrate striated muscles

无脊椎动物和脊椎动物横纹肌肌球蛋白亚片段 2 区域的表征

• 发表时间: 2008
• 作者: Y. Ochiai;G. F. Wang;S;Watabe
• 通讯作者: Watabe

Characterization of decapodiformes myosin heavy chains: Structure, function and evolution International Conference of Comparative Biochemistry and Physiology

十足目肌球蛋白重链的表征：结构、功能和进化 国际比较生物化学和生理学会议

• 发表时间: 2007
• 作者: Y. Ochiai;G.F. Wang;Y. Ono;S. Watabe
• 通讯作者: S. Watabe