Physiological and molecular biological studies on acquisition of auditory responsiveness in zebrafish

斑马鱼听觉反应能力获得的生理和分子生物学研究

• 批准号: 22300126
• 负责人: ODA Yoichi
• 金额: $ 10.65万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010-04-01 至 2013-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22300126/
• 关键词: 内耳; 有毛細胞; 耳石; 球形嚢; 卵形嚢; マウスナー細胞; カリウムチャネル; ゼブラフィッシュ; 網様体脊髄路ニューロン; キンギョ; 聴覚; 触覚; 逃避運動; 機械受容; 発火パターン

The developmental origin of inner ear hair cells, role of ear stone size on acoustic sensitivity and molecular mechanisms underlying developmental change in firing properties of hindbrain neurons were studied in vivo in zebrafish embryos and larvae. We demonstrated first that tether cells, a few ciliated cells associated with an ear stone (otolith) in the embryonic inner ear, morphologically and functionally differentiate into first hair cells. Second, the saccule inner ear organ containing a large otolith is necessary to detect sound, whereas the utricle containing a small otolith is not. But, utricle hair cells with experimentally enlarged otoliths acquired the sense of sound, suggesting that otolith size is crucial to sense sound. Third, unique firing properties of Mauthner cells among the hindbrain reticulospinal neurons receiving auditory nerve inputs are acquired during early development and coexpression of Kv beta subunits with low-threshold Kv1 subunits is required for them.

在斑马鱼胚胎和幼鱼体内研究了内耳毛细胞的发育起源、耳石大小对声敏感性的作用以及后脑神经元放电特性发育变化的分子机制。我们首先证明了系绳细胞（与胚胎内耳中的耳石（耳石）相关的一些纤毛细胞）在形态和功能上分化为第一个毛细胞。其次，含有大耳石的球囊内耳器官是检测声音所必需的，而含有小耳石的椭圆囊则不是。但是，通过实验扩大耳石的椭圆囊毛细胞获得了声音的感觉，这表明耳石的大小对于感知声音至关重要。第三，接受听觉神经输入的后脑网状脊髓神经元中的 Mauthner 细胞在早期发育过程中获得了独特的放电特性，并且它们需要 Kv β 亚基与低阈值 Kv1 亚基的共表达。

项目成果

Glycinergic transmissions are required for formation and maintenance of synaptic glycine receptor clusters in zebrafish Mauthner cells in vivo

斑马鱼莫特纳细胞体内突触甘氨酸受体簇的形成和维持需要甘氨酸能传递

• 发表时间: 2012
• 作者: Iori Yamanaka;Mariko Miki;Kazuhide Asakawa;Koichi Kawakami;Yoichi Oda;Hiromi Hirata
• 通讯作者: Hiromi Hirata

Large-otolith-mediated mechanotransduction and afferent neural transmission in saccular pathway underlie auditory perception in larval zebrafish

囊状通路中大耳石介导的机械转导和传入神经传递是斑马鱼幼虫听觉感知的基础

• 发表时间: 2012
• 作者: Maya Inoue;Masashi Tanimoto;Yoichi Oda
• 通讯作者: Yoichi Oda

名古屋大学生物第七講座

名古屋大学生物学第7课程

マウスナー細胞の活動に伴うゼブラフィッシュ仔魚の逃避運動の可塑的変化

斑马鱼幼虫逃逸运动的塑性变化与毛特纳细胞活性相关

• 发表时间: 2013
• 作者: Okimoto H;Tanaka S;Araki H;Ohashi E;Tsurimoto T.;高橋めぐみ,井上摩耶,谷本昌志,小田洋一
• 通讯作者: 高橋めぐみ,井上摩耶,谷本昌志,小田洋一

Common sensory inputs and differential outputs of segmentally homologous reticulospinal neurons in zebrafish hindbrain.

斑马鱼后脑节段同源网状脊髓神经元的常见感觉输入和差分输出。

• 发表时间: 2013
• 作者: Mishiro A;Tanimoto M;Oda Y
• 通讯作者: Oda Y