Development and Establishment of Photosensory System in the Larvel and Young Fish

幼鱼光感系统的发育和建立

基本信息

批准号：
09660199
负责人：
OMURA Yuji
金额：
$ 2.11万
依托单位：
NAGOYA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1999
项目状态：
已结题

项目摘要

Our research results from 1997 to 1999 are summarized as follows.(1) Although it has been presumed that the retina of the leptocephalus of the European eel possesses a homogenous pattern of rod-like photoreceptors, we found the cone-like photoreceptor cells in the retina of the eel leptocephalus collected from the Pacific Ocean close to the spawning area and also from the new born eel larvae cultivated in laboratory aquarium. The electron microscopic study also revealed that the retina of larval eel possesses regular cone-like photoreceptor cells at least during the early stage of their development, as same as the other pelagic fish (Omura et al. 1997a).(2) The development of the inner ear, especially sensory maculae and otoliths was studied in the ayu plecoglossus altivelis and rainbow trout Oncorhychus mykiss, using light and electron microscopy. Consequently, it was suggested that fishes, while the initiation and oscillation of the daily increment formation may be under circadian en … More docrine control in the rainbow trout (Salem & Omura, 1998a, b).(3) Immunolocalization of taurine was investigated in the pineal organ and retina of an anadromous fish Plecoglossus altivelis, in the developing retina of a lefteye flounder paralichthys olivaceus, and also between the dark- and light- adapted retinas of the glass eel young goldfish. Intense immunostaining was found in the outer plexiform layer, but not in the outer segments of rod cells. Consequently, it was suggested that the lack of immunoreactivity in the rod outer segment may depend on light stimulation (Omura et al. 1997b, 1999, Omura & Yoshida 1999, Omura & Inagaki 2000).(4) Field potential responses to local electrical stimulation were analyzed in slices of goldfish optic tectum in vitro. Slowly developing potentiation in goldfish retino-tectal synapses is masked by prior stimulation, suggesting an involvement of glutamatergic field excitatory post-synaptic potential. An involvement of NMDA receptors and a possible involvement of adenosine were also examined (Ishikawa & Matsushima 1997, Ishikawa et al. 1997, 1999). Less

我们1997年至1999年的研究成果总结如下：(1)虽然人们推测欧洲鳗鱼的头颅视网膜具有同质的杆状感光细胞，但我们在欧洲鳗鱼的头颅视网膜中发现了锥状感光细胞。从靠近产卵区的太平洋以及实验室培养的新生鳗鱼幼体中采集的细头鳗的视网膜电子显微镜研究还表明，鳗鱼幼体的视网膜至少在其发育的早期阶段具有规则的圆锥状感光细胞，与其他中上层鱼类一样（Omura et al. 1997a）。(2)使用光和技术，研究了香鱼 plecoglossus altivelis 和虹鳟鱼 Oncorhychus mykiss 的内耳发育，特别是感觉斑和耳石。电子显微镜检查表明，在虹鳟鱼中，每日增量形成的起始和振荡可能受到昼夜节律控制（Salem & Omura，1998a，b）。（3）牛磺酸的免疫定位在溯河产卵鱼 Plecoglossus altivelis 的松果体器官和视网膜以及左眼比目鱼发育中的视网膜中进行了研究牙鲆，以及玻璃鳗幼金鱼的暗适应和光适应视网膜之间，在外丛状层中发现了强烈的免疫染色，但在视杆细胞的外节中没有发现，这表明缺乏。视杆外节的免疫反应性可能取决于光刺激（Omura et al. 1997b, 1999, Omura & Yoshida 1999, Omura & Inagaki 2000）。（4）在体外金鱼视顶盖切片中分析了对局部电刺激的场电位反应，金鱼视网膜顶盖突触中缓慢发展的增强被先前的刺激所掩盖，表明谷氨酸能场兴奋性突触后的参与。还检查了 NMDA 受体的参与和腺苷的可能参与（Ishikawa & Matsushima 1997，石川等人，1997，1999）。