Analysis of molecular mechanisms of neuropathic pain and neciceptive signaling

神经性疼痛和伤害性信号传导的分子机制分析

• 批准号: 21658096
• 负责人: OHTA Toshio
• 金额: $ 2.16万
• 依托单位: Tottori University (2010-2011)Hokkaido University (2009)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21658096/
• 关键词: 薬理; 疼痛; Caイメージング; TRPA1; 知覚神経細胞; 疼痛反応; 炎症性疼痛; ヒスタミン; TRPチャネル; 知覚神経

To elucidate functional significance of Transient Receptor potential V1(TRPV1) and TRPA1 which are expressed in sensory neurons as neuropathic and inflammatory pain signaling, fluorescent protein-fused expressing vectors were constructed and heterologously expressed in mammalian cells. Their functions were compared to those obtained from sensory neurons. Histamine and hydrogen sulfide, which are synthesized and released under inflammatory conditions, potenciated and stimulated these channels, and elicited pain signaling through the activation of TRPV1 and TRPA1, respectively. These results suggest that TRP channels are important molecular targets for pain-regulation.

为了阐明在感觉神经元中表达的瞬时受体电位 V1 (TRPV1) 和 TRPA1 作为神经病理性和炎症性疼痛信号传导的功能意义，构建了荧光蛋白融合表达载体并在哺乳动物细胞中异源表达。将它们的功能与从感觉神经元获得的功能进行比较。在炎症条件下合成和释放的组胺和硫化氢会增强和刺激这些通道，并分别通过激活 TRPV1 和 TRPA1 引发疼痛信号传导。这些结果表明 TRP 通道是疼痛调节的重要分子靶点。

项目成果

Molecular Cloning and Functional Characterization of Xenopus tropicalis Frog Transient Receptor Potential Vanilloid 1 Reveal Its Functional Evolution for Heat, Acid, and Capsaicin Sensitivities in Terrestrial Vertebrates

• DOI: 10.1074/jbc.m111.305698
• 发表时间: 2012-01-20
• 期刊: JOURNAL OF BIOLOGICAL CHEMISTRY
• 影响因子: 4.8
• 作者: Ohkita, Masashi;Saito, Shigeru;Ohta, Toshio
• 通讯作者: Ohta, Toshio

Xenopus Tropicalis TRPV1遺伝子クローニングと発現・機能解析

热带爪蟾TRPV1基因克隆、表达及功能分析

• 发表时间: 2010
• 作者: 大北真嗣;齋藤茂;今川敏明;高橋賢次;太田利男
• 通讯作者: 太田利男

Decreased calcium channel currents and facilitated epinephrine release in the calcium channel beta3 subunit-null mice.

钙通道β3亚基缺失小鼠中钙通道电流减少并促进肾上腺素释放。

• 发表时间: 2010
• 作者: 村上学;大場貴喜;根本隆行;柳田俊彦;太田利男;M.MURAKAMI
• 通讯作者: M.MURAKAMI

Facilitated epinephrine release in the calcium channel ss3 subunit-null mice

促进钙通道 ss3 亚基缺失小鼠中肾上腺素的释放

• 发表时间: 2011
• 作者: 齋藤茂;大北真嗣;曽我部隆彰;門脇辰彦;太田利男;富永真琴;小川ひとみ;村上学
• 通讯作者: 村上学

温度感受性TRPチャネルの多様性および機能進化

温度敏感TRP通道的多样性和功能进化

• 发表时间: 2011
• 作者: 齋藤茂;大北真嗣;曽我部隆彰;門脇辰彦;太田利男;富永真琴
• 通讯作者: 富永真琴