Analysis of novel collectin, CL-K1 function in colorectal cancer

新型集合素、CL-K1在结直肠癌中的功能分析

• 批准号: 21790640
• 负责人: MOTOMURA Wataru
• 金额: $ 2.75万
• 依托单位: Asahikawa Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21790640/
• 关键词: コレクチン; 大腸癌; 腫瘍マーカー; 消化器癌; CL-K1

The present study aimed to determine CL-K1 expression in tumourigenesis of human gastrointestinal (GI) malignancies. We examined CL-K1 distribution in normal human and cancer tissues by using tissue arrays. We also examined CL-K1 distribution in stomach and colorectal tissue and performed immunohistochemical analyses to determine its expression in epithelial and connective tissues. Real-time PCR and immunohistochemical analyses demonstrated that CL-K1 expression in humans resembles that in mice, and in human cancer tissues, CL-K1 expression tends to be elevated. Our examination of 42 human gastric tissues showed that CL-K1 protein expression was frequently observed in gastric cancer tissues compared with that in adjacent normal mucosa. In addition, in 98 human colorectal tissues, CL-K1 expression showed a significant gradual increase with progression to malignancy. Moreover, CL-K1 expression tended to be higher in invasive cancer tissues than in non-invasive cancer tissues. CL-K1 was expressed in the proximal tubules in the kidneys, vascular portion of several organs, hepatocytes in the liver, and mucosa in the gastrointestinal tract. It is of interest that the CL-K1 expression profile in humans is similar to that in mice. In a study of 140 speciments of GI tract tissues, CL-K1 up-regulation correlated with malignancy. Together, these histological and pathological findings may be useful in furthering our understanding of biological functions in cancer tissues.

本研究旨在确定人类胃肠道（GI）恶性肿瘤的肿瘤发生中的CL-K1表达。我们通过使用组织阵列检查了正常人和癌组织中的CL-K1分布。我们还检查了胃和结肠直肠组织中的CL-K1分布，并进行了免疫组织化学分析，以确定其在上皮和结缔组织中的表达。实时PCR和免疫组织化学分析表明，人类中的Cl-K1表达类似于小鼠和人类癌组织中的CL-K1表达往往会升高。我们对42种人体胃组织的检查表明，与邻近的正常粘膜相比，在胃癌组织中经常观察到Cl-K1蛋白的表达。此外，在98个人类结直肠组织中，Cl-K1表达随着恶性肿瘤的发展而显着逐渐增加。此外，在侵入性癌组织中，CL-K1表达往往高于非侵入性癌组织。 Cl-K1在肾脏的近端小管中表达，几个器官的血管部分，肝脏中的肝细胞和胃肠道中的粘膜。令人感兴趣的是，人类中的Cl-K1表达谱类似于小鼠。在对140个胃肠道组织标本的研究中，CL-K1上调与恶性肿瘤相关。总之，这些组织和病理学发现可能有助于进一步了解我们对癌症组织中生物学功能的理解。