The relationship of the inflammatory infiltrating cell in prostate grand and chemokine

前列腺炎性浸润细胞与趋化因子的关系

• 批准号: 13671660
• 负责人: ITOH Naoki
• 金额: $ 2.24万
• 依托单位: Sapporo Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671660/
• 关键词: prostate; inflammatory cell; chemokine; IL-8; Gatifloxacin(GFLX)

(1)The pathological study of inflammatory cell infiltration in the prostate in patientsPatients entered into the study received radical cystoprostatectomy because of invasive bladder cancer in our institute from 1998 to 2000. The infiltrating cell type was assessed by immunostaining using a series of antibodies CD2O, CD45RO, CD68,S-100. The types of infiltrating cells were dominantly T cells. Inflammatory cell infiltration was seen in 32.4% cases.(2)Antimicrobial agents and suppression of cytokine secretion of prostate cancer cell line.PC-3,a human prostate cancer cell lines, was used for the experiment. IL-8 concentrations of the supematants were increased depending on the concentrations of Mycoplasma hominis. The mRNA of Toll like receptor-2 and Toll like receptor4 were demonstrated by RT-PCR in PC-3 cell lines. The activities of NF-κB were increased depending on the concentration of Mycoplasma hominisa. These data show that the signals from Toll-like receptor up the activities of NF-κB and lead to produce of IL-8. The influence of GFLX on IL-8 mRNA expression was analyzed using RT-PCR. GFLX significantly attenuated the TNF-α induced IL-8 mRNA level. GFLX suppresses the secretion of IL-8 from PC-3 in a dose-dependent manner. The results may imply that GFLX has an anti-inflammatory effect on chronic prostatitis.

(1)患者前列腺炎性细胞浸润的病理学研究 1998年至2000年在我所因浸润性膀胱癌接受根治性膀胱前列腺切除术的患者。使用CD2O系列抗体进行免疫染色评估浸润细胞类型，浸润细胞类型以CD45RO、CD68、S-100为主。 32.4%的病例中出现了这种情况。(2)抗菌剂和抑制前列腺癌细胞系的细胞因子分泌。实验使用人前列腺癌细胞系PC-3，上清液的IL-8浓度根据情况增加。 RT-PCR 证实了 PC-3 细胞系中 Toll 样受体 2 和 Toll 样受体 4 的浓度对 NF-κB 活性的影响。这些数据表明来自Toll样受体的信号增强了NF-κB的活性并导致IL-8的产生。使用RT-PCR分析GFLX对IL-8 mRNA表达的影响显着减弱。 TNF-α 诱导的 IL-8 mRNA 水平以剂量依赖性方式抑制 PC-3 的 IL-8 分泌。该结果可能暗示 GFLX 对慢性病具有抗炎作用。前列腺炎。

项目成果

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Masumori N、Tsukamoto T 等人：“男性下尿路症状的自然史 - 纵向社区的结果 -”泌尿学。

伊藤直樹: "前立腺肥大症(BPH)"診断と治療. 90. 1051-1056 (2002)

伊藤直树：“BPH”诊断和治疗。90。1051-1056（2002）。

Kunishima Y, Matsukawa M, Takahashi S, Itoh N, Tsukamoto T, et al.: "National institutes of health chronic prostatitis symptom index for Japanese men"Urology. 60. 74-77 (2002)

Kunishima Y、Matsukawa M、Takahashi S、Itoh N、Tsukamoto T 等：“国立卫生研究院日本男性慢性前列腺炎症状指数”泌尿科。

Tsukamoto T, Masumori N, Itoh N: "BPH-Other medical therapies."Voiding Disorders Digest(in Japanese). 9. 66-70 (2001)

Tsukamoto T、Masumori N、Itoh N：“BPH-其他医学疗法。”排尿障碍文摘（日语）。

Tsukamoto T, Masumori N, Itoh N: "Benign prostatic hyperplasia."Rinsho Seijinbyo(in Japanese). 31. 195-200 (2001)

Tsukamoto T、Masumori N、Itoh N：“良性前列腺增生。”Rinsho Seijinbyo（日语）。