Role of bacteriophage communities in paddy soils and their gene diversity and uniqueness

水稻土中噬菌体群落的作用及其基因多样性和独特性

基本信息

批准号：
21380046
负责人：
KIMURA Makoto
金额：
$ 11.23万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2009
资助国家：
日本
起止时间：
2009 至 2011
项目状态：
已结题

项目摘要

The role of bacteriophage(phage) communities in paddy soils was studied from the phage contribution to bio-element cycling in this study, and the frequency of phage infection to their hosts and the carbon flow of plant residue to phage DNA were evaluated. Fresh and dried^13C-labelled callus cells were used as the model materials of root cap cells and plant residue, respectively. The gene diversity and uniqueness of phage communities in soils were examined from the phage g23, g20 and psbA sequences in comparison with those in marine and freshwater environments. The frequency of virulent phage infection to bacteria in soils was markedly higher than those reported from marine and freshwater environments. The recovery of 13C-labelled g23 and g20 clones indicated that the phage communities are important in bio-element cycling as the drivers of the microbial loop. The diversity of g23 clones was significantly higher than those in marine and freshwater environments, and their sequences were mostly soil-specific and different from those of marine and freshwater origins. In addition, it was known that g20 and psbA gene sequences in the floodwater of paddy fields were more similar to the sequences in the freshwater environment than to those in the marine environments and that g20 sequences in paddy field soils were different from those not only of marine and freshwater origins but also in the floodwaters overlying.

在这项研究中，研究了噬菌体（噬菌体）群落在稻田中的作用，从噬菌体贡献对生物元素循环的贡献，以及对宿主的噬菌体感染频率以及植物残留物的碳流向噬菌体DNA的流动。新鲜和干燥的^13C标记的愈伤组织分别用作根盖细胞和植物残基的模型材料。与海洋和淡水环境中的噬菌体G23，G20和PSBA序列相比，从噬菌体G23，G20和PSBA序列中检查了土壤中噬菌体群落的基因多样性和独特性。土壤中毒性噬菌体感染的频率明显高于海洋和淡水环境中报道的频率。 13C标记的G23和G20克隆的恢复表明，作为微生物环的驱动因素，噬菌体群落在生物元素循环中很重要。 G23克隆的多样性明显高于海洋和淡水环境中的克隆，它们的序列主要是土壤特异性的，与海洋和淡水起源的序列不同。此外，众所周知，稻田洪水中的G20和PSBA基因序列与淡水环境中的序列更相似，而与海洋环境中的序列相似，而帕迪田土壤中的G20序列与不仅与那些不仅不同于海洋和淡水的起源，但也在洪水泛滥。

项目成果

期刊论文数量（0）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Bacterial communities incorporating carbon of root cap cells under upland soil conditions

高地土壤条件下吸收根冠细胞碳的细菌群落

DOI：
发表时间：
2011
期刊：
影响因子：
0
作者：
Yong Li;Chol Gyu Lee;Takeshi Watanabe;Jun Murase;Susumu Asakawa;and Makoto Kimura
通讯作者：
and Makoto Kimura

Changes in major capsid genes (g23) of T4-type bacteriophages with soil depth in two Japanese rice fields

DOI：
10.1007/s00374-009-0362-2
发表时间：
2009-02
期刊：
Biology and Fertility of Soils
影响因子：
6.5
作者：
Guanghua Wang;J. Murase;K. Taki;Yoshinori Ohashi;N. Yoshikawa;S. Asakawa;M. Kimura
通讯作者：
Guanghua Wang;J. Murase;K. Taki;Yoshinori Ohashi;N. Yoshikawa;S. Asakawa;M. Kimura

ファージ感染が土壌細菌の計数に及ぼす影響

噬菌体感染对土壤细菌计数的影响