Establishment of immuno-monitoring system for the infectious disease control and analysis of neutrophil dysfunction in various compromised hosts

传染病控制免疫监测系统的建立及各类受损宿主中性粒细胞功能障碍的分析

• 批准号: 21591300
• 负责人: ONO Yasuo
• 金额: $ 2.66万
• 依托单位: Teikyo University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21591300/
• 关键词: 感染症防御学; 易感染性; 好中球; セプシス; 遊走能; 細胞膜抗原; 遺伝子発現; 活性酸素産生能; 易感染性宿主; TLR; 細胞内遺伝子発現; HL-60; CXCR1遺伝子導入; 活性酸素; 細胞表面抗原; MAPK; 基準値

Neutrophil chemotaxis was reduced in septic patients and poorly controlled diabetic patients. Neutrophil bactericidal ability in the patients with liver cirrhosis, SLE, AIDS, myeloma, severe burn and elderly persons was significantly lower than that of healthy controls. Expression levels of neutrophil membrane antigens, CD14 and TLR-4 were higher in patients with sepsis ; however, the expression levels of CD11b and CD16 were lower. The gene expression of TREM-1 in neutrophils associated with the severity of sepsis. Analysis of neutrophil functions using these assays may be available as the sensitive and reliable immuno-monitoring tools for determining the capacity of host defense against bacterial infections in compromised hosts.

化粪池患者和控制糖尿病患者的嗜中性粒细胞趋化性降低。肝硬化，SLE，AIDS，骨髓瘤，严重烧伤和老年人的嗜中性粒细胞杀菌能力明显低于健康对照组。败血症患者的中性粒细胞膜抗原，CD14和TLR-4的表达水平较高。但是，CD11b和CD16的表达水平较低。与败血症严重程度相关的中性粒细胞中Trem-1的基因表达。使用这些测定法分析中性粒细胞功能可以作为敏感且可靠的免疫监测工具，用于确定宿主防御损害宿主中细菌感染的能力。

项目成果

Enhancement of interleukin-8-induced chemotactic response and reactive oxygen species production in HL-60 cells expressing CXCR1

• DOI: 10.1007/s10156-011-0321-3
• 发表时间: 2012
• 期刊: Journal of Infection and Chemotherapy
• 影响因子: 2.2
• 作者: Takane Kikuchi-Ueda;S. Tansho;Y. Ono

Evaluation of TREM1 gene expression in circulating polymorphonuclear leukocytes and its inverse correlation with the severity of pathophysiological conditions of patients with acute bacterial infections

急性细菌感染患者循环多形核白细胞TREM1基因表达评估及其与病理生理状况严重程度的负相关

• 发表时间: 2012
• 期刊: Jap J Infect Dis
• 作者: Ubagai T;Tansho-Nagakawa S;Ikeki T;Ono Y
• 通讯作者: Ono Y

会長シンポジウム[ヒト・微生物・薬剤関係からみた感染症、最近の知見]1.宿主(ヒト)からみた感染症

主席座谈会【人类、微生物、药物视角下的传染病，最新发现】1.宿主（人类）视角下的传染病

• 发表时间: 2010
• 作者: Goto H;斧康雄
• 通讯作者: 斧康雄

教育講演1 好中球からみたセプシス

教育讲座1 从中性粒细胞的角度看脓毒症

• 发表时间: 2009
• 作者: Nieuwenhuis EES;Matsumoto T;斧康雄
• 通讯作者: 斧康雄

真菌および細菌由来菌体成分の好中球MAPK familyリン酸化に対する影響

真菌和细菌细胞成分对中性粒细胞 MAPK 家族磷酸化的影响

• 发表时间: 2011
• 作者: 越尾修;ほか
• 通讯作者: ほか