Risk assessments of the emerging fish pathogen in the environmental condition of rising water temperature.

水温上升环境条件下新出现的鱼类病原体的风险评估。

• 批准号: 21580229
• 负责人: YOSHIDA Terutoyo
• 金额: $ 3万
• 依托单位: University of Miyazaki
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21580229/
• 关键词: C群レンサ球菌; Streptococcus dysgalactiae; 高水温; ブリ; カンパチ; 魚病; 抗原; 薬剤感受性; レンサ球菌; ブリ属魚類; streptococcus dysgalactiae; ランスフィールドC群; 抗原型; コンゴーレッド

TH-CR(Todd-Hewitt Congo red) agar was developed to detect and presumptively identify the GCSD(Group C Streptococcus dysgalactiae) obtained from artificially or naturally infected fish. Typical symptoms were observed in artificially infected fish at higher temperature(28°C), thus need an attention at the high water temperature season. The genetic analysis revealed that the fish isolates were genetically very similar to each other with high DNA. DNA relatedness and sequence homology. Seventeen isolates in GCSD collected Asian countries were found to be resistant to oxytetracycline and carried the tet(M) gene, except for the strains collected in Taiwan and the PP1564 strain collected in China. The amino acid sequence of SOF was identical among fish isolates. A primer set targeting the sof-FD gene was designed and applied to a PCR assay for discriminating fish isolates from mammalian isolates.

TH-CR（托德-休伊特刚果红）琼脂用于检测和推测鉴定从人工或自然感染的鱼中获得的 GCSD（C 型停乳链球菌），在较高温度（28°C）下，在人工感染的鱼中观察到典型症状。 ，因此在高水温季节需要注意。遗传分析显示，这些鱼类在遗传上非常相似，DNA 相关性和序列也很高。 GCSD 收集的亚洲国家的 17 个分离株被发现对土霉素具有抗性并携带 tet(M) 基因，除了台湾收集的菌株和中国收集的 PP1564 菌株外，SOF 的氨基酸序列在鱼类中是相同的。设计了针对 sof-FD 基因的引物组，并将其应用于 PCR 测定，以区分鱼类分离株和哺乳动物分离株。

项目成果

Homogeneity of Streptococcus dysgalactiae from farmed amberjack Seriola dumerili in Japan

• DOI: 10.1007/s12562-010-0243-5
• 发表时间: 2010-07-01
• 期刊: FISHERIES SCIENCE
• 影响因子: 1.9
• 作者: Nishiki, Issei;Noda, Masahiro;Yoshida, Terutoyo
• 通讯作者: Yoshida, Terutoyo

ブリ属魚類由来Streptococcus dysgalactiaeの薬剤感受性

黄尾鱼停乳链球菌的药物敏感性

• 发表时间: 2009
• 期刊: 日本水産学会誌 (印刷中)
• 作者: 西木一生;野本竜平;米村輝一朗;中西健二;平江多績;村瀬拓也;伊丹利明;吉田照豊
• 通讯作者: 吉田照豊

Surface properties of Streptococcus dysgalactiae strains isolated from marine fish Bull.

从海鱼公牛中分离出的停乳链球菌菌株的表面特性。

• 发表时间: 2009
• 期刊: Fish Pathol
• 影响因子: 0.6
• 作者: M. Abdelsalam;S. C. Chen and T. Yoshida
• 通讯作者: S. C. Chen and T. Yoshida

Dissemination of streptococcal pyrogenetic exotoxin G(spegg) with an IS-like element in fish isolates of Streptococcus dysgalactiae

具有 IS 样元素的链球菌热生外毒素 G（spegg）在停乳链球菌鱼分离株中的传播

• 发表时间: 2010
• 期刊: FEMS Microbiology Letters
• 影响因子: 2.1
• 作者: M. Abdelsalam;S. C. Chen and T. Yoshida
• 通讯作者: S. C. Chen and T. Yoshida

Homogeneity of Streptococcus dysgalactiae isolated from amberjack, Seriola dumerili, in Japan

日本从鰤鰤分离的停乳链球菌的同质性

• 发表时间: 2010
• 期刊: Fisheries Science
• 影响因子: 1.9
• 作者: I. Nishiki;M. Noda;T. Itami and T. Yoshida
• 通讯作者: T. Itami and T. Yoshida