Analysis of protective antigen and virulence factors of Lactococcus garvieae, fish bacterial pathogen

鱼类病原菌加维乳球菌的保护性抗原及毒力因子分析

基本信息

批准号：
12660176
负责人：
YOSHIDA Terutoyo
金额：
$ 2.18万
依托单位：
Miyazaki University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

项目摘要

ABSTRACT : Strains of Lactococcus garvieae were examined for the presence of cell surface structures. Two types of cell capsular strains such as highly developed capsule (KG9408) and micro-capsule (MS93003) of L. garvieae were found, carrying fimbrie-like components, projecting from cell surface. While one stain (NSS9319) has neither cell capsular nor fimbrie-like structures on its cell surface. Highly developed cell capsular strain indicated higher virulence to fish than that of micro-capsular or non-capsular strain. Bacteriphage susceptibilities to these three strains were clearly distinguished from KG9408 to MS93003 and NSS9310 strains. Immune protection against Lactococcus garvieae infection was induced in yellowtail, Seriola guinqueradiata immunized with formalin-killed L. garvieae KG9408 and MS93003. However immune protection was also induced by immunization of NSS9310, the level of protection was significantly lower than that of KG9408 andMS93003. protection was induced by immunization of NSS9310. Passive immunization with yellowtail immune sera raised against KG9408 and MS93003 conferred strong protection to yellowtail with the rapid bacterial clearance after the infection of L. garvieae. Immunoblotting analysis of proteins extracted from L. garvieae strains using rabbit anti-KG9408 and MS93003 serum, and yellowtail anti-KG9408 and MS93003 serum indicated that some bands from NSS9310 were not detected as seen in KG9408 and MS93003 strains.

摘要：检查了加维乳球菌菌株的细胞表面结构的存在。发现了两种类型的细胞荚膜菌株，例如L. garvieae高度发达的荚膜（KG9408）和微胶囊（MS93003），携带从细胞表面突出的菌毛状成分。而一种染色剂 (NSS9319) 的细胞表面既没有细胞荚膜，也没有菌毛状结构。高度发育的细胞荚膜菌株对鱼类的毒力高于微荚膜或非荚膜菌株。 KG9408、MS93003 和 NSS9310 菌株对这三种菌株的噬菌体敏感性明显不同。用福尔马林灭活的加维乳球菌 KG9408 和 MS93003 免疫的鰤鱼、鰤鱼，诱导了针对加维乳球菌感染的免疫保护。但NSS9310免疫也诱导了免疫保护，但保护水平显着低于KG9408和MS93003。通过NSS9310的免疫诱导保护。使用针对KG9408和MS93003的黄尾鱼免疫血清进行被动免疫，可以对黄尾鱼提供强有力的保护，在感染L. garvieae后细菌能够快速清除。使用兔抗 KG9408 和 MS93003 血清以及黄尾鱼抗 KG9408 和 MS93003 血清对从 L. garvieae 菌株中提取的蛋白质进行免疫印迹分析，结果表明未检测到 NSS9310 的一些条带，如 KG9408 和 MS93003 菌株中所见。