Effects of oocyte activation factors derived from spermatozoa on the embryo development.

精子来源的卵母细胞激活因子对胚胎发育的影响。

• 批准号: 12660263
• 负责人: HOSOI Yoshihiko
• 金额: $ 1.09万
• 依托单位: Kinki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12660263/
• 关键词: Intracytoplasmic Sperm Injection; Activation factor; Rabbit Oocyte; Calcium oscillation; Cynomologus Monkey; Additional stimulation; Electric Stimulation; 卵子活性化条件; 胚発生; 精子由来活性化因子; 活性化能力; ハムスター精子抽出液; 発生能力の改善

During fertilization, sperm entry triggers a series of intracellular calcium oscillations critical to oocyte activation. This activation is prerequisite to the resumption and completion of meiosis, subsequent pronuclear formation and DNA synthesis.Intracytoplasmic sperm injection (ICSI) has been applied to human infertility treatment. This technique could be also useful for determining the roles of various sperm components in fertilization. Although ICSI is usually a highly successful treatment for severe male factor infertility, some cases repeatedly experience low ICSI fertilization rates (<50%). Possible causes include failure of oocyte activation resulting from sperm and/or oocyte defects. Previous studies have shown that oocytes injected with round spermatids (Hosoi et al., 2000) can be activated by the calcium ionophore A23187.Various artificial treatments mimic sperm-triggered events and induce parthenogenetic development in MII oocytes. It suggests that additional stimulation helps development of fertilized eggs under insufficient condition. In considering the application of intracytoplasmic sperm injection (ICSI) for endangered species and infertility patients, in many cases only not adequate gametes are expected to be used for reproduction. In conclusion, our study showed that oocytes activation factors was very important for artificial fertilization.

在受精过程中，精子进入会引发一系列对卵母细胞激活至关重要的细胞内钙振荡。这种激活是减数分裂恢复和完成、随后的原核形成和DNA合成的先决条件。胞浆内单精子注射(ICSI)已应用于人类不育症的治疗。该技术还可用于确定各种精子成分在受精中的作用。尽管 ICSI 通常是治疗严重男性因素不孕症的一种非常成功的治疗方法，但有些病例反复出现 ICSI 受精率低（<50%）的情况。可能的原因包括精子和/或卵母细胞缺陷导致卵母细胞活化失败。先前的研究表明，注射圆形精子细胞的卵母细胞（Hosoi et al., 2000）可以被钙离子载体 A23187 激活。各种人工处理模拟精子触发事件并诱导 MII 卵母细胞的孤雌发育。这表明额外的刺激有助于在条件不足的情况下受精卵的发育。在考虑将胞浆内单精子注射（ICSI）应用于濒临灭绝的物种和不孕不育患者时，在许多情况下，预计仅使用不充足的配子进行繁殖。总之，我们的研究表明卵母细胞激活因子对于人工受精非常重要。

项目成果

細井美彦: "不妊治療におけるクローン関連技術の利用"産婦人科の世界. 55巻2号. 47-52 (2003)

Yoshihiko Hosoi：“克隆相关技术在不孕症治疗中的应用”《妇产科世界》第 55 卷，第 2. 47-52 期（2003 年）。

細井美彦 他2名: "サルES細胞株の樹立"実験医学. 19・15. 16-22 (2001)

Yoshihiko Hosoi等2人：“猴ES细胞系的建立”实验医学19・15（2001）。

細井美彦: "新しい産科学(分担執筆)"名古屋大学出版会. 283 (2002)

细井芳彦：《新产科》（撰稿人）名古屋大学出版社 283（2002）。

Y.Hosoi et al.: "Establishment of embryonic stem cell lines from Cynomolgus Monkey blastocysts produced by IVF or ICSI"Developmental Dynamics. 222. 273-279 (2001)

Y.Hosoi 等人：“通过 IVF 或 ICSI 产生的食蟹猴囊胚建立胚胎干细胞系”发育动力学。

Y.Hosoi et al.: "Fertilization by intracytoplasmic sperm injection and subsequent embryo development in vitro to blastoc"Journal of Mammalian Ova Research. 20巻1号(Inprint). (2003)

Y.Hosoi 等人：“通过胞浆内精子注射受精并随后在体外使胚芽发育”，《哺乳动物卵子研究杂志》第 20 卷，第 1 期（Inprint）。