Evaluation of environmental factors in atopic dermatitis patients by quantitative assay for Th2 adjuvant activity

Th2佐剂活性定量测定评价特应性皮炎患者的环境因素

• 批准号: 20710026
• 负责人: HIGASHI Takehiro
• 金额: $ 2.83万
• 依托单位: Saitama Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20710026/
• 关键词: 環境影響評価; 樹状細胞; アジュバント; アトピー性皮膚炎; 母乳

The skin of a patient with Atopic dermatitis (AD) is abnormally and easily affected by irritants, food, and environmental allergens. Results from observational birth cohort studies, case-control studies, and one cluster randomized intervention trial have generally failed to demonstrate a protective effect of breastfeeding on outcomes of atopic dermatitis, allergic sensitization, wheezing, or asthma. Although these studies reported that AD correlates with breast-feeding, it is unclear whether mothers' milk itself contributes to AD development. The magnitude of Th2 adjuvant activity is associated with increased levels of cAMP in PMA-derived THP-1 cells. The short incubation period (10 min) for this assay allows for the testing of non-sterile materials such as live bacteria, or non-sterile milk, as reported in the current study.Our study showed that some mothers, whose children developed AD within 6 months after birth, carry high Th2 adjuvant activity in their milk obtained 4 days right after birth. Such activity was not observed in mothers' milk fed to healthy infants. The current results suggest a direct effect of mothers' milk with the development of AD.

特应性皮炎（AD）患者的皮肤异常且容易受到刺激性，食物和环境过敏原的影响。观察性出生队列研究，病例对照研究和一项簇随机干预试验的结果通常未能证明母乳喂养对特应性皮炎的结局的保护作用，过敏症，喘息或哮喘。尽管这些研究报告说AD与母乳喂养相关，但尚不清楚母亲的牛奶本身是否有助于AD的发展。 Th2辅助活性的大小与PMA衍生的THP-1细胞中CAMP的水平增加有关。该测定法的短孵育周期（10分钟）允许在当前的研究中报告的非菌株材料，例如活细菌或非菌群牛奶。在喂给健康婴儿的母亲的牛奶中未观察到这种活性。当前的结果表明，母亲的牛奶随着AD的发展而直接影响。

项目成果

Dopamine released by dendritic cells polarizes Th2 differentiation

• DOI: 10.1093/intimm/dxp033
• 发表时间: 2009-06-01
• 期刊: INTERNATIONAL IMMUNOLOGY
• 影响因子: 4.4
• 作者: Nakano, Kazuhisa;Higashi, Takehiro;Matsushita, Sho
• 通讯作者: Matsushita, Sho

Dopamine Dl-like receptor antagonist, SCH23390, exhibits apreventive effect on diabetes mellitus that occurs naturally in NOD mice.

多巴胺 D1 样受体拮抗剂 SCH23390 对 NOD 小鼠中自然发生的糖尿病具有预防作用。

• 发表时间: 2009
• 期刊: Biochem.Biophvs.Res.Commun. 383
• 作者: M.A.Rahman;H.Hasegawa;K.Kadohashi;T.Maki;K.Ueda;Hashimoto K
• 通讯作者: Hashimoto K

アトピー性皮膚炎患児摂取母乳の試験管内アジュバント活性評価法を用いたコホート研究

特应性皮炎儿童摄入母乳体外佐剂活性评价方法的队列研究

• 发表时间: 2008
• 作者: 平野勇二郎;稲村實;外岡秀行;井村秀文;牧輝弥;東丈裕;牧輝弥;東丈裕
• 通讯作者: 東丈裕

Human Th17 cell clones and natural immune responses

人类 Th17 细胞克隆和自然免疫反应

• 发表时间: 2008
• 期刊: Allergol. Int. 57(2)
• 作者: Matsushita;S
• 通讯作者: S

ドパミンD1様受容体アンタゴニストはNODマウスにおける糖尿病の自然発症を抑制する

多巴胺 D1 样受体拮抗剂抑制 NOD 小鼠糖尿病的自发发展

• 发表时间: 2009
• 作者: 青木洋介;永田正喜;曲渕裕樹;福岡麻美;橋本久実子;冨岡治明;青木洋介;橋本久実子
• 通讯作者: 橋本久実子