Analysis on the molecular mechanisms of wider dynamic range and higher time-resolution in cone photoreceptor cells.

锥体感光细胞更宽动态范围和更高时间分辨率的分子机制分析。

• 批准号: 20700350
• 负责人: TACHIBANAKI Shuji
• 金额: $ 2.75万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20700350/
• 关键词: 視細胞; 錐体; GRK; Sモジュリン; 順応; RGS9; cGMP; Sモミュリン・s26; 発色団代謝経路; ALOL反応

In vertebrate retina, there are two types of photoreceptor cells, called rods and cones. The time resolution of cone mediated vision is higher than that of rod-mediated vision. This higher time-resolution depends on shorter responses of cones. It is also known that the range of light-intensity that cone can mediate is wider than that of rod. In this study, the underlying mechanisms of cone-specific responses are studied. The results show that the lifetime of activated enzymes, working in the phototransduction mechanism, are shorter in cones than in rods. And It is also revealed that the enzymatic activities in the phototransduction mechanism are more highly modulated in cones than in rods. These findings show molecular bases of the cone specific photoresponses.

在脊椎动物视网膜中，有两种类型的感光细胞，称为棒和锥。锥体介导的视力的时间分辨率高于杆介导的视力。这种较高的时间分辨率取决于锥体的较短响应。众所周知，锥体可以介导的光强度范围比杆的范围宽。在这项研究中，研究了锥体特异性反应的潜在机制。结果表明，在光转导机制中起作用的活化酶的寿命比圆锥体短。而且还揭示了光转导机制中的酶活性在锥体中比在杆上更高度调节。这些发现表明了锥体特异性光的分子碱基。

项目成果

Highly efficient retinal metabolism in cones

• DOI: 10.1073/pnas.0806593105
• 发表时间: 2008-10-14
• 期刊: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• 影响因子: 11.1
• 作者: Miyazono, Sadaharu;Shimauchi-Matsukawa, Yoshie;Kawamura, Satoru
• 通讯作者: Kawamura, Satoru

2種類の視物質キナーゼ(GRK1,GRK7)の活性の違いをもたらす領域の特定

鉴定导致两种视觉物质激酶（GRK1、GRK7）活性差异的区域

• 发表时间: 2009
• 作者: 瀬野亜希;竹本訓彦;橘木修志(発表者);河村悟;橘木修志
• 通讯作者: 橘木修志

Amino Acid Residues in GRK1/GRK7 Responsible for Interaction with S-modulin/Recoverin

GRK1/GRK7 中负责与 S-调节蛋白/恢复蛋白相互作用的氨基酸残基

• 期刊: Photochemistry and Photobiology (in press)
• 作者: Torisawa;A.;Arinobu;D;Tachibanaki;S.;Kawamura;S
• 通讯作者: S

Rod and cone photoreceptors : molecular basis of the difference in their さphysiology

视杆细胞和视锥细胞光感受器：生理学差异的分子基础

• 发表时间: 2008
• 期刊: Comparative Biochemistry and Physiology, Part A 150(4)
• 作者: Kawamura;S. and Tachibanaki;S.
• 通讯作者: S.

錐体特異的なGT活性化と不活性化をもたらす分子基盤

锥体特异性 GT 激活和失活的分子基础

• 发表时间: 2008
• 作者: Mikio Kataoka;H.iroshi Nakagawa;有信大輔・橘木修志・河村悟;竹本訓彦・松川淑恵・橘木修志・河村悟;橘木修志・越谷祐貴・河村悟
• 通讯作者: 橘木修志・越谷祐貴・河村悟