Investigation for establishment of periodontal regenerative therapy using stem cells in cultured periodontal ligament cells

利用培养牙周膜细胞干细胞建立牙周再生治疗的研究

• 批准号: 20592436
• 负责人: KOBAYASHI Makoto
• 金额: $ 3万
• 依托单位: Showa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20592436/
• 关键词: ヒト歯根膜; 間葉系幹細胞; 歯周組織再生; Side Population(SP)細胞; 骨分化制御; BMPアンタゴニスト; twist; biglycan; decorin; 培養ヒト歯根膜細胞; SP細胞; 組織幹細胞; 腱・靱帯マーカー; ヒト歯根膜細胞; Side Population 細胞; 腱・靭帯マーカー分子

In 2008 : The various types of stem cells exist in side population(SP) effused strongly Hoechst 33342 dye. The SP cells in bone marrow express cell surface markers of human stem cells(HSCs) and are highly enriched for HSC activity. In this study, we clarified the characteristics of SP cells isolated from human periodontal ligament(HPDL) cells. Unfortunately, the present data concluded that HPDL cells contain SP cells, whereas the SP cells are not enriched for mesenchymal stem cell(MSC)-like properties.In 2009, Twist and bone morphogenetic protein(BMP) antagonists may play an important role in maintenance of constancy of PDL, by which negatively regulate osteoblastic differentiation of cells existing in PDL. In this study, changes of these molecules expression by inducing osteoblastic differentiation were assessed in HPDL cells. We further investigated regulatory effects of BMP antagonists on osteoblastic differentiation in the cells. The present results suggest that under physiological … More condition, twist and BMP antagonists ; gremlin and follistatin, which are constitutively expressed in HPL cells, negatively regulate osteoblastic differentiation of the cells. Further, the induction of osteoblastic differentiation in HPL cells simultaneously enhances twist expression, while it suppresses expression of gremlin and follistatin, as the result, the osteoblastic differentiation may be modulated.PDL contains fibroblastic cells, which may participate in formation and maintenance of PDL fibers, and osteoblastic cells with a potential to generate alveolar bone and cementum, however, the specific markers for recognition and isolation of each cell type have not been clarified. The purpose of this study was to isolate alkaline phosphatase(ALP)-positive and-negative subpopulations from HPDL cells using flowcytometry and to identify functional difference between the 2 subsets in terms of mitogenic activity, osteogenic potential and expression of decorin as well as biglycan. The present results suggest that ALP-positive HPDL cells have osteoblast-like phenotype, while ALP-negative HPDL cells are fibroblastic cell population with weak osteogenic potential. Further, biglycan may positively regulate osteoblastic differentiation in ALP-positive HPDL cells, while decorin, whose expression is increased by osteogenic induction, may negatively regulate mineralization in both subpopulations.In 2010, to investigate characteristics of MSC existed in PDL, CD271+/CD90+cells, which were isolated from HPDL by FACS, were single cell cultured, and the clones with MSC-like properties were collected. Therefore, CD271+/CD90+may be a marker to collect MSC from HPDL. Less

在2008年：侧面种群中的各种干细胞（SP）强烈地染料33342染料。阐明了来自人类牙周韧带（HPDL）细胞的SP细胞的特征在这项研究中，在PDL中维持PDL eoblastic差异的重要作用。 ST在生理上……更多的条件，扭曲和BMP拮抗剂； follistatin，成骨细胞的分化可能是模块化的。PDL包含成纤维细胞，可能参与PDL纤维的形成和维持，而成骨细胞具有产生肺泡骨和骨质的潜力，具有特定的标记，以识别每个细胞类型，并且具有隔离性具有识别的离子和隔离。已经澄清了酸盐的目的是使用HPDL细胞的碱性磷酸盐（ALP）阳性和阴性亚挑选，并鉴定MIT OGENIC活性的2个子集的功能差异结果表明，阳性细胞具有o糖母细胞型表型，而ALP阴性HPDL细胞具有弱的成骨潜力。 MSC的存在于PDL中，CD271+/CD90+细胞培养了MSC样性能的克隆，因此CD271+/CD90+可能是从HPDL收集MSC的标记

项目成果

Characteristics of side cell population cells existing in periodontal ligament cells

牙周膜细胞中存在的侧细胞群细胞的特征

• 发表时间: 2008
• 作者: Koide Y;Kobayashi M;et al.
• 通讯作者: et al.

ヒト歯根膜細胞中に存在するアルカリフォスファターゼ陽性細胞と陰性細胞の特徴

人牙周膜细胞碱性磷酸酶阳性和阴性细胞的特征

• 发表时间: 2009
• 期刊: Dental Med Res 29(1)
• 作者: 鶴見亜有子、小林誠;ら
• 通讯作者: ら

ヒト歯根膜細胞中のアルカリフォスファターゼ陰性細胞の特徴

人牙周膜细胞碱性磷酸酶阴性细胞的特征

• 发表时间: 2008
• 作者: 鶴見亜有子;小林誠;奥津朋彦;ら
• 通讯作者: ら

培養ヒト歯根膜細胞におけるTwistと各種BMP antagonistの発現制御

Twist 和各种 BMP 拮抗剂在培养的人牙周膜细胞中表达的调节

• 发表时间: 2008
• 期刊: Dental Med Res
• 作者: 森高俊一郎;小林誠;三井将ら;(2番目)
• 通讯作者: (2番目)